胶原酶和自体血注入法建立脑出血动物模型的比较  

Comparison of intracerebral hemorrhage animal models established by injecting collagenase and autologous blood

作　　者：张波[1] 孙善全[1] 邱国平[1] 甘胜伟[1] 朱淑娟[1] 钟小燕[1] 伍修宇[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆400016

出　　处：《解剖学杂志》2012年第1期75-78,103,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30470608）

摘　　要：为脑出血发病机制和／或干预性治疗的模型选择提供参考。方法：采用胶原酶和自体血注入大鼠尾状核建立脑出血（ICH）模型，运用磁共振成像（MRI）、干湿重法、伊文思蓝（EB）测定法、免疫荧光和免疫印迹分别比较两组脑出血模型的血肿体积、脑含水量（BWC）、血脑屏障（BBB）通透性和水通道蛋白4（AQP4）的表达变化。结果：两组脑出血模型在MRI、BWC、EB含量和AQP4的表达变化均存在差异。结论：自体血模型是较为接近临床的脑出血动物模型。To provide reference for study of pathologic mechanism and/or intervening therapy of intracerebral hemorrhage （ICH）. Methods： Collagenase and autologous blood were injected into the caudate nucleus to establish ICH models in rats. The hematoma volume was measured by magnetic resonance imaging. The changes of brain water content （BWC） were measured by wet and dry weight methods. Evan＇s blue （EB） was used to measure the integrity of blood-brain bar- tier （BBB）. The changes of AQP4 expression were detected by immunofluorescence,and the contents of AQP4 were detected by West- em blotting. Results： The changes of hernatorm volume, BWC, EB contents and AQP4 expression between the two ICH models showed a significant difference. Conclusion： Autologous blood animal model is relatively close to clinical ICH conditions.

关 键 词：脑出血 动物模型 胶原酶 自体血 水通道蛋白4 大鼠 

分 类 号：R285.5[医药卫生—中药学]