高血压循环内皮祖细胞变化和动脉弹性的关系  

Relationship between Alteration of Circulating Endothelial Progenitor Cells and Arterial Elasticity in Patients with Essential Hypertension

作　　者：杨震[1] 夏文豪[1] 罗初凡[2] 张媛媛[1] 苏晨[1] 陈龙[1] 廖新学[1] 陶军[1] 

机构地区：[1]中山大学附属第一医院．高血压血管病科广东广州510080 [2]中山大学附属第一医院心血管介入病区,广东广州510080

出　　处：《中山大学学报（医学科学版）》2012年第1期54-59,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(u0732002,30800215,30770895);广东省自然科学基金博士启动项目(8451008901000793);广州市科技计划项目(2007Z3-E0241)

摘　　要：【目的】探讨高血压患者循环内皮祖细胞(EPC)数量和功能变化与动脉弹性的关系。【方法】检测20例高血压患者和21例年龄匹配血压正常者的大动脉弹性(C1)和小动脉弹性(C2)。同时用流式细胞仪测定测定CD34和KDR双标阳性循环内皮祖细胞水平,ac-LDL及lectin荧光标记方法评估体外培养内皮祖细数量,检测内皮祖细胞的迁移和增殖能力。【结果】与血压正常者相比,高血压患者C1和C2减退,循环内皮祖细胞迁移和增殖功能明显下降,但是两组患者内皮祖细数量无明显差别。循环内皮祖细胞迁移或增殖功能和C1或C2呈明显正相关,多元回归分析显示循环内皮祖细胞迁移或增殖功能是C1或C2的独立预测因素。【结论】高血压动脉弹性下降与循环内皮祖细胞功能受损密切相关,提示内源性血管内皮修复能力下降是高血压血管损伤的重要机制。[ Objective] The study was to investigate the association between alteration in circulating endothelial progenitor cells （EPC） and hypertension-related reduced arterial elasticity. [ Methods ] We measured the artery elasticity profiles including C 1 large and C2 small artery elasticity indices in the patients with essential hypertension （n = 20） and age-matched normotensive subjects （n= 21 ）. We also performed flow cytometry analysis to evaluate the number of CD34 and KDR double-positive labeled circulating EPC before and after exercise, and acetylated-LDL and lectin fluorescent staining method to evaluate the number of cultured EPC. In addition, the EPC migratory and proliferative activities were measured. [ Results ] Compared with normotensive subjects, the patients with hypertension exhibited decreased C1 large and C2 small artery elasticity indices. The number of circulating EPC was not different between the two groups. The migratory and proliferative activities of circulating EPC in hypertensive patients were lower than those in normotensive subjects. Both proliferatory and migratory activities of circulating EPC closely correlated with C1 large and C2 small artery elasticity indices. Multivariate analysis identified both proliferative and migratory activities of circulating EPC as independent predictors of C1 large and C2 small artery elasticity indices. [Conclusion] The present study demonstrates for the first time that impaired activity of circulating EPC is associated with reduced arterial elasticity in patients with hypertension. The fall in endogenous repair capacity of vascular endothelium may be an important mechanism underlying hypertension-related vascular injury.

关 键 词：高血压 内皮祖细胞 动脉弹性 血管损伤 

分 类 号：R541.4[医药卫生—心血管疾病]