脂肪干细胞与小肠黏膜下层构建仿生骨膜的体内成骨  

In vivo osteogenesis of bionic periosteum constructed by adipose-derived stem cells seeded onto small intestinal submucosa

作　　者：张开刚[1] 张月东[1] 王玫[2] 

机构地区：[1]泰安市中心医院骨科,山东省泰安市271000 [2]泰山医学院,山东省泰安市271016

出　　处：《中国组织工程研究与临床康复》2012年第3期426-430,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:脂肪干细胞与小肠黏膜下层两者具有良好的组织相容性,但将二者复合构建仿生骨膜修复骨缺损的效果如何尚需进一步验证。目的:探讨以小肠黏膜下层为支架材料复合成骨诱导的脂肪干细胞构建仿生骨膜,体内成骨的可行性。方法:取兔腹股沟区的脂肪分离培养脂肪干细胞经成骨诱导后,与猪小肠黏膜下层复合体外培养3周,构建仿生骨膜。将仿生骨膜埋置于裸鼠皮下,并以小肠黏膜下层复合未成骨诱导的脂肪干细胞的复合材料置于裸鼠皮下做对照。结果与结论:兔脂肪干细胞经成骨诱导后与小肠黏膜下层体外复合培养,见两者黏附良好,细胞分泌大量的细胞外基质。植入4,8,12周组织学和透射电镜观察见有大量骨组织形成,未成骨诱导材料复合物组内无成骨细胞。仿生骨膜植入体内8周免疫组织化学测试骨钙蛋白、骨桥蛋白呈阳性反应。提示仿生骨膜植入裸鼠体内后可形成具有良好血运的新生骨组织。BACKGROUD：Both adiposederived stem cells（ADSCs） and small intestinal submucosa（SIS） have good biocompatibility.But the effects of bionic periosteum composite constructed by them on bone defects repairment need further investigation.OBJECTIVE：To study the osteogenic feasibility of bionic periosteum constructed by ADSCs seeded onto SIS in vivo.METHODS：After osteogenic induction,ADSCs isolated from the rabbits inguinal fat were co-cultured with porcine SIS in vitro for 3 weeks to build bionic periosteum.Bionic periosteum was embedded in nude mice.ADSCs without osteogenesis induction seeded onto SIS were also embedded in nude mice as control.RESULTS AND CONCLUSION：After osteogenic induction,ADSCs were well adhered onto the SIS and a large amount of extracellular matrix was found in cells.At 4,8 and 12 weeks after grafting,there was a large amount of bone formation detected by immunohistochemistry and transmission electron microscope.There was no cortical bone formation in the non-induced composite group.At 8 weeks after bionic periosteum grafting,immunohistochemistry results of osteopontin and osteocalcin were positive.It is indicated that bionic periosteum transplanted into nude mice can form new bone with good blood supply.

关 键 词：仿生骨膜 脂肪干细胞 小肠黏膜下层 裸鼠 组织工程 

分 类 号：R318[医药卫生—生物医学工程]