氟转化涂层镁合金材料与诱导后人骨髓间充质干细胞的相容性  

Cellular biocompatibility of induced human bone marrow stromal cells to fluoride conversion coating magnesium alloys

作　　者：姜海英 闫征斌[1,2] 张照[3] 艾红军[4] 

机构地区：[1]大庆市油田总医院口腔科,黑龙江省大庆市163001 [2]佳木斯大学口腔医学院,黑龙江省佳木斯市154002 [3]广州体育职业技术学院医务所,广东省广州市510650 [4]中国医科大学附属口腔医院口腔修复科,辽宁省沈阳市110002

出　　处：《中国组织工程研究与临床康复》2012年第3期459-462,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：辽宁省自然科学技术基金(2008079)~~

摘　　要：背景:氟转化涂层的AZ31B镁合金和β-磷酸三钙涂层的AZ31B镁合金是中科院金属研究所新研制的镁合金,是否具有良好的生物相容性尚不确切。目的:以诱导的人骨髓间充质细胞作为检测细胞,评价氟转化涂层的AZ31B镁合金和β-磷酸三钙涂层的AZ31B镁合金材料的细胞相容性。方法:实验分为镁合金AZ31B组,氟转化涂层的AZ31B镁合金组和β-磷酸三钙涂层的AZ31B镁合金组。以诱导的人骨髓间充质细胞作为检测细胞,分别取3种材料浸提液进行体外细胞相容性实验。结果与结论:氟转化涂层的AZ31B镁合金和β-磷酸三钙涂层的AZ31B镁合金材料细胞毒性分级均为1级,镁合金AZ31B材料细胞毒性分级为2级。提示氟转化涂层的AZ31B镁合金和β-磷酸三钙涂层的AZ31B镁合金材料生物相容性优于未经处理镁合金AZ31B材料。BACKGROUND：As newly developed magnesium alloy,whether fluoride conversion coating and β-tricalcium phosphate（β-TCP） coating Biodegradable AZ31B magnesium alloy has better cell compatibility is not confirmed.OBJECTIVE：To evaluate the cell compatibility of fluoride conversion coating and β-TCP coating Biodegradable AZ31B magnesium alloy tested with the induced human bone marrow stromal cells.METHODS：The experiment was divided into three groups：AZ31B magnesium alloy group,fluoride conversion coating AZ31B magnesium alloy group and β-TCP coating Biodegradable AZ31B magnesium alloy group.The induced human bone marrow stromal cells were regarded as the tested cells,and the extracts of the three materials were used in the compatibility experiments in vitro.RESULTS AND CONCLUSION：The toxicity of cells in the leaching liquor of fluoride conversion coating and β-TCP coating Biodegradable AZ31B magnesium alloys were Grade 1.The toxicity of cells in the leaching liquor of AZ31B magnesium alloys was Grade 2.This experiment had proved that the fluoride conversion coating and β-TCP coating Biodegradable AZ31B magnesium alloys were superior than AZ31B magnesium alloys.

关 键 词：镁合金 生物医用材料 骨髓间充质干细胞 Β-磷酸三钙 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]