PCR、SPA-ELISA、GDT3种方法检测猪伪狂犬病毒的效果比较  

Comparation of the effects for PRV detection by using PCR,SPA-ELISA and GDT methods

作　　者：侯静[1] 周静[1] 杨泽晓[1] 姚学萍[1] 王印[1,2] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《黑龙江畜牧兽医》2012年第3期99-101,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：教育部长江学者和创新团队发展计划创新团队项目(IRT 0848);四川农业大学双支计划项目(00270401)

摘　　要：为了比较临床中常用的猪伪狂犬病诊断方法的检测效果,试验采用常规方法对猪伪狂犬病毒进行不同浓度梯度的稀释,分别通过聚合酶链式反应(PCR)、葡萄球菌A蛋白-酶联免疫吸附试验(SPA-ELISA)、琼脂扩散试验(GDT)3种方法对猪伪狂犬病毒进行检测,并对各种方法的敏感性、可重复性、经济性、简便性进行综合分析。结果表明:SPA-ELISA法和PCR法的灵敏度都远远高于GDT法,并且二者的敏感性相当;PCR法和SPA-ELISA法适用于实验室对PRV的确诊;GDT法所耗费的材料较少、经济、操作简便,适合于临床大规模的检测和初次检测猪伪狂犬病毒的存在。Different concentration gradients of the Pseudorabies virus dilution were detected by using PCR,SPA-ELISA,GDT methods,then the sensitivity,repeatability,economy and simplicity of the three test results were compared and analysized.The results showed that PCR and SPA-ELISA with the similar higher sensitivity than GDT method,were suitable for Pseudorabies virus detection in laboratory,but GDT cost less materials,economic,simplicity.It is more suitable for the initial detection and large-scale diagnosis of the presence of Pseudorabies virus in the clinic trials.

关 键 词：猪伪狂犬病毒 PCR法 SPA—ELISA法 GDT法 

分 类 号：S854.43[农业科学—临床兽医学]