魟鱼软骨多糖对小鼠恶性黑色素移植瘤MMP-9表达的影响  

Effects of RCG on the Expression of MMP-9 in C57BL/6 Mice with Malignant Melanoma

作　　者：孙岩[1] 郭斌[2] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《中国药房》2012年第11期967-969,共3页China Pharmacy

基　　金：辽宁省自然科学基金项目(201102132)

摘　　要：目的:研究魟鱼软骨多糖(RCG)对小鼠恶性黑色素移植瘤基质金属蛋白酶-9(MMP-9)表达的影响。方法:体外培养黑色素瘤细胞(B16),接种B16细胞于C57BL/6小鼠右侧腋下部位,复制小鼠黑色素瘤模型。实验分为生理盐水(等容生理盐水)、环磷酰胺(CTX,60mg·kg-1)和魟鱼软骨多糖高、中、低剂量(700、300、100mg·kg-1)组,灌胃给药,每天1次,连续21d。观察肿瘤生长情况,计算原发瘤抑制率;采用Westernblot方法检测肿瘤组织中MMP-9的蛋白表达情况;采用RT-PCR方法检测MMP-9的基因表达情况。结果:与生理盐水组比较,魟鱼软骨多糖高、中、低剂量组小鼠原发瘤抑制率显著升高(P<0.01);魟鱼软骨多糖高、中、低剂量组MMP-9蛋白表达、基因表达显著降低(P<0.01)。结论:魟鱼软骨多糖能明显抑制小鼠黑色素瘤原发瘤的生长,可能与其抑制肿瘤MMP-9的蛋白表达和MMP-9mRNA的表达有关。OBJECTIVE：To study the effects of Ray cartilage glycosaminoglycans （RCG） on the expression of MMP-9 in mice with malignant melanoma. METHODS： B16 tumor cells were cultivated in vitro, then inoculated in right oxter of the C57BL/6 mice. The malignant melanoma model was established. The experimental mice were randomly divided into normal saline group （constant volume normal saline）, CTX group （CTX, 60 mg·kg ^-1） and RCG high-dose, medium-dose and low-dose groups （700, 300, 100 mg·kg ^-1）. Those groups were given medicine intragastrically once a day for consecutive 21 days. The growth of tumor was observed, and the inhibitory rate of tumor was measured. The protein expression of MMP-9 in tumor was determined with Western blot, and the expression of MMP-9 mRNA was detected with RT-PCR. RESULTS： Compared with normal saline group, inhibitory rates of primary tumor in RCG high-dose, medium-dose and low-dose groups increased significantly （P〈0.01）, the protein expression level of MMP-9 and the gene expression level of MMP-9 in RCG groups were lowered significantly（P〈0.01）. CONCLUSION： RCG could effectively inhibit the growth of malignant melanoma, which is probably related to that RCG inhibit the protein expression of MMP-9 and MMP-9 mRNA.

关 键 词：魟鱼软骨多糖 恶性黑色素移植瘤 基质金属蛋白酶-9 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]