甘草甜素对大鼠体内苦参碱药动学的影响  

Effects of Glycyrrhizin on the Pharmacokinetics of Matrine in Rats

作　　者：漆林艳[1] 黄亮[1] 杨宝春[1] 潘弟仪[1] 卢文芬[1] 陈志良[1] 

机构地区：[1]南方医科大学南方医院药学部科研组,广州510515

出　　处：《中国药房》2012年第11期985-986,共2页China Pharmacy

摘　　要：目的:研究甘草甜素对大鼠体内苦参碱药动学的影响。方法:采用高效液相色谱法测定大鼠血浆中苦参碱的含量,色谱柱为Diamonsil C1(8250mm×4.6mm,5μm),流动相为乙腈-0.1%H3PO(4三乙胺调pH值至8;35∶65),流速为1mL·min-1,柱温为30℃,检测波长为220nm。实验分为2组,A组灌胃苦参碱(70mg·kg-1),B组灌胃苦参碱(70mg·kg-1)和甘草甜素(70mg·kg-1)。应用3p97软件处理苦参碱的血药浓度-时间数据。结果:A组Cmax=(6.861±0.635)mg·L-1,AUC0～t=(47.105±7.062)mg·h·L-1,tmax=(1.471±0.438)h;B组Cmax=(4.122±0.965)mg·L-1,AUC0～t=(35.507±5.024)mg·h·L-1,tmax=(1.158±0.175)h。经统计学分析,2组之间Cmax、AUC、CL、t1/2α、t1/2β、K21均有显著性差异(P<0.05)。结论:苦参碱与甘草甜素合用,与其单独给药比较,甘草甜素对其在大鼠体内的吸收、分布、代谢及排泄都有不同程度的影响。OBJECTIVE： To study the effect of glycyrrhizin （GL） on the pharmacokinetics of matrine （M） in rats. METHODS： The content of M in rat plasma was determined by HPLC method. Diamonsil C18（250 mm×4.6 mm, 5 μm） column was adopted with mobile phase consisted of acetonitrile-0.1% HaPO4（pH value adjusted to 8 using triethylamine, 35：65） at the flow rate of 1 mL·min-1. The column temperature was 30 ℃ and detection wavelength was set at 220 mn. Rats were divided into 2 groups, i.e. group A was given M （70 mg·kg^-1） intragastrically and group B was given M （70 mg·kg^-1） and GL mixture （70 mg·kg^-1） intragastrically. The plasma concentration-time data of M were analyzed by 3P97 software. RESULTS： In group A, Cmax was （6.861 ± 0.635） mg·L^-1, AUC0-t was （47.105 ± 7.062） mg·h·L^-1,tmax was （1.471 ± 0.438）h; in group B, Cmax was （4.122 ± 0.965）mg·L^-1, AUC0-t, was （35.507 ± 5.024）mg·h·L^-1,tmax was （1.158 ± 0.175）h. Cmax,AWC,CL,t1/2α、t1/2β, K21 were significantly different between 2 groups （P〈0.05）. CONCLUSION： Compared with M alone, GL has an impact on the absorption, distribution, metabolism and excretion of M under M mix with GL.

关 键 词：高效液相色谱法 苦参碱 甘草甜素 药动学 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]