RP-HPLC法测定杜仲中杜仲醇的含量  

Content Determination of Eucommiol in Eucommia ulmoides by RP-HPLC

作　　者：巩卓[1] 王耀欣[1] 陈鑫[1] 冯硕[1] 车庆明[1] 韩南银[1] 

机构地区：[1]北京大学药学院,北京100191

出　　处：《中国药房》2012年第11期1023-1024,共2页China Pharmacy

摘　　要：目的:建立测定杜仲中杜仲醇含量的方法。方法:采用反相高效液相色谱法。色谱柱为Chromasail C18(250mm×4.6mm,5μm),流动相为甲醇-水(8∶92),流速为1mL·min-1,柱温为25℃,检测波长为210nm。结果:杜仲醇进样量在0.4676～5.8450μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为99.04%,RSD=2.16%(n=9)。结论:本方法简单、稳定、可控性强、重复性好,可用于杜仲中杜仲醇的含量测定。OBJECTIVE： To develop the method for the content determination of eucommiol in Eucommia ulmoides. METH- ODS： RP-HPLC method was adopted. The sample was separated on Chromasail C18（250 mm×4.6 mm, 5 μm） column with methanol-water （8：92） as the mobile phase at the flow rate of 1 mL·min^-1. The column temperature was 25 %and the detection wavelength was set at 210 nm. RESULTS： The linear range of eucommiol was 0.467 6-5.845 0μg（r=0.999 9） with an average recovery of 99.04% （RSD=2.16%, n=9）. CONCLUSION： The method is simple, stable, controllable and repeatable, and it could be used for the content determination of encommiol in Eucommia ulmoide.

关 键 词：杜仲 杜仲醇 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]