鸡GPC1基因外显子8的SNPs与部分免疫性状的相关性分析  

Association between Single Nucleotide Polymorphisms of Exon 8 of GPC1 Gene and Immune Traits of Chicken

作　　者：盛中伟[1] 常国斌[1] 栾德琴[1] 周伟[1] 胡国顺[1] 刘艳[1] 李芹[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国畜牧杂志》2012年第5期1-6,共6页Chinese Journal of Animal Science

基　　金：江苏省自然科学基金(BK2009190);江苏省科技支撑计划(BE2010375)

摘　　要：采用PCR-SSCP对12个不同鸡种glypican-1(GPC1)基因外显子8的单核苷酸多态性进行检测,同时检测如皋鸡、安卡鸡和文昌鸡3个鸡种56日龄的5个免疫性状,进一步分析SNP位点与免疫性状的关系。结果表明:12个鸡种中均出现3种带型,存在g.155 C>A错义突变,引起苏氨酸突变为精氨酸。12个鸡种群中,AA、AB和BB基因型平均频率分别为0.259、0.545和0.211;平均观察杂合度为0.466,平均Shannon信息指数为0.671。除乌骨鸡、大骨鸡偏离了Hardy-Weinberg平衡外(P<0.05),其余群体均处平衡状态。基于该位点构建的UPGMA聚类图将12个鸡种分为3大类,反映了12个鸡种在抗性方面存在的差异。经过关联分析,如皋、安卡鸡中AB型个体的IL-1浓度、H/L值与BB型存在显著差异,与AA型差异不显著;在其他免疫性状中各基因型之间均无显著差异。安卡鸡的一般抗性总体低于如皋鸡和文昌鸡。The PCR-SSCP technology and DNA sequencing was conducted to detect single nucleotide polymorphisms （SNPs） of exon 8 in glypican-1 （GPC1） gene in twelve breeds. Five immune traits and their associations with SNPs were also detected at 56 days of age in Wenchang, Rugao and Anka chickens. 1 non-synonymous SNP, g.155 C〉A, were found, resulting in amino acid sequence altered from Threonine to Arginine. In all 12 breeds, the average frequencies of AA, AB and BB genotypes were 0.259, 0.545, and 0.211, respectively, and the average value of observed heterozygosis and Shannon＇s index were 0.4658 and 0.6710. Except Taihe chicken and Dagu chicken, the other populations were all in Hardy-Weinberg equilibrium at this locus （P〉0.05）. The dendrograms divided the 12 chicken populations into 3 clusters, which reflected the resistance difference between these 12 breeds. IL-1 concentrations and H / L values of AB genotype individuals was significantly differ from that of BB genotype individuals, rather than that of AA genotype individuals in Rugao and Anka chickens. There was no significance in SRBC antibody titer, ND antibody and AI antibody titer between three genotypes. In general, the disease resistance of Rugao and Wenchang were better than that of Anka chicken.

关 键 词：GPC1基因 单核苷酸多态性 免疫性状 鸡 

分 类 号：S831.2[农业科学—畜牧学]