大鼠骨骼肌挫伤后外周血源性纤维细胞的数量变化  

The Number of Circulating Fibrocytes of Skeletal Muscle in Rats after Contusion

作　　者：李力强[1] 于天水[1] 官大威[1] 范琰琰[1] 赵锐[1] 马文翔[1] 张书韬[1,2] 张舒[1] 任鹏[1] 李骄勇[1,3] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001 [2]黑龙江省公安厅,黑龙江哈尔滨150008 [3]伊春市公安局,黑龙江伊春153000

出　　处：《法医学杂志》2012年第1期1-5,11,共6页Journal of Forensic Medicine

基　　金：教育部高等学校博士学科点专项科研基金项目(200801590020)

摘　　要：目的探讨大鼠骨骼肌挫伤后损伤区外周血来源的纤维细胞数量变化规律。方法制作大鼠骨骼肌挫伤模型,应用CD45、procollagenⅠ双重免疫荧光染色法检测大鼠骨骼肌挫伤后各时间点外周血来源的纤维细胞的变化。结果对照组大鼠骨骼肌中未见CD45、procollagenⅠ阳性的纤维细胞分布;损伤组大鼠骨骼肌挫伤后6 h～1 d,仅见少数CD45阳性的单个核细胞;伤后3 d,开始有少量CD45、procollagenⅠ阳性的纤维细胞聚集于损伤区;伤后5d,阳性的纤维细胞数量明显增加;伤后7d达高峰,此后逐渐减少,同时还发现相对于3 d组,纤维细胞的体积随着时间延长逐渐变大;伤后14 d,纤维细胞胞质中的CD45和pro-collagenⅠ的表达强度都相应减弱。结论大鼠骨骼肌挫伤后,挫伤区外周血来源的纤维细胞的数量变化具有一定的时间规律性,可望作为推断骨骼肌挫伤时间的指标之一。Objection To investigate the time-dependent appearance of circulating fibrocytes of skeletal muscle in rats after contusion.Methods The model of skeletal muscle wound was established in rat.The circulating fibrocytes in contused skeletal muscle were detected by CD45 and procollagen Ⅰ double immunofluorescence staining method.Results In the control group,CD45-and procollagen Ⅰ-positive cells were not detected in skeletal muscle.A few CD45 cells were observed aged from 6 h to 1 d after contusion.A few CD45-and procollagen Ⅰ-positive cells（fibrocytes） initially gathered in injury area 3 d after injury.The ratio of positive fibrocytes significantly increased 5 d after injury.The ratio of fibrocytes was highest at 7 d after contusion and then decreased.The volume of fibrocytes showed bigger with injury time increase compared with 3 d group.The expression of procollagen Ⅰ and CD45 were weakened at 14 d after injury.Conclusion The circulating fibrocytes are detected in contused skeletal muscle in time-dependent pattern.Circulating fibrocytes may be a marker in the wound age determination for contused skeletal muscle.

关 键 词：法医病理学 创伤和损伤 肌 骨骼 纤维细胞 损伤时间 大鼠 

分 类 号：DF795.1[医药卫生—法医学]