用AFM研究静电纺丝负载药物的缓释机制  

Drug Release Mechanism of BSA-loaded Electrospun Fibers by AFM

作　　者：徐芃[1,2] 李伟[1,3] 周华从[1,2] 刘会洲[1] 

机构地区：[1]中国科学院过程工程研究所绿色过程与工程实验室,北京100190 [2]中国科学院研究生院,北京100049 [3]首都师范大学化学系,北京100048

出　　处：《过程工程学报》2012年第1期119-124,共6页The Chinese Journal of Process Engineering

摘　　要：研究了壳聚糖(CS)-聚环氧乙烯(PEO)和聚乳酸(PLA)-CS载药电纺纤维的牛血清蛋白(BSA)释放行为.利用原子力显微镜(AFM)观察了纤维在模拟体液中的分解与溶胀行为.CS-PEO纤维在模拟体液中分解10h时BSA总释放量高达96%,1d后纤维结构基本分解完全,在缓冲液中浸泡120min后纤维直径由浸泡10min时的0.727μm增大到1.43μm,纤维表面发生明显分解.CS-PEO和PLA-CS纤维具有不同的药物释放机制,CS-PEO载药纤维的药物释放主要是纤维结构快速分解,BSA扩散作用相对较弱;而后者在溶液中没有明显分解,浸泡30d后纤维直径从浸泡1d时的1.9μm增至2.8μm,BSA释放量不到总量的50%,其药物释放机制可能主要依靠BSA的扩散.Experiments were carried out to study the drug delivery mechanism of bovine serum albumin （BSA） loaded chitosan （CS）-poly（ethylene oxide） （PEO） nanofibers and poly（L-lactic acid） （PLA）-CS nanofibers. Atomic force microscope （AFM） was used to observe the degradation and swelling behavior of fibers in PBS solution. As seen from the SEM images of scaffolds and drug release profiles, their BSA delivery mechanisms were different. 96% BSA was released from CS-PEO nanofibers after 10 h and the structure was almost totally degraded in 1 d. AFM images showed a distinct degradation process of CS-PEO fiber and fber diameter increasing from 0.727 to 1.43 μm from 10 to 120 min. So BSA releasing was mainly controlled by the rapid degradation of CS-PEO fibers. On the contrary, no obvious degradation was observed in the AFM or SEM images of PLA-CS nanofibers. The fiber diameter increased from 1.9 to 2.8 μm from 1 to 30 d, and the amount of BSA in solution was less than 50%. It demonstrated that the BSA delivery of PLA-CS fibers depended on the drug diffusion process.

关 键 词：原子力显微镜 静电纺丝 药物缓释 牛血清蛋白 

分 类 号：TS157[轻工技术与工程—纺织材料与纺织品设计]