硒、锰在培养大鼠心肌细胞上的抗氧化损伤作用  

ANTIOXIDATIVE DAMAGE EFFECT OF SE AND MN ON CULTURED CARDIAC MUSCLE CELL IN RAT

作　　者：钟国赣[1] 张保国[1] 张文杰[1] 岳刚[1] 江岩 

机构地区：[1]白求恩医科大学 [2]河北医学科学院

出　　处：《中国地方病防治》1990年第3期130-133,共4页Chinese Journal of Control of Endemic Diseases

摘　　要：向培养基中加入黄嘌呤-黄嘌呤氧化酶(X-XOD),诱发超氧阴离子自由基,造成心肌细胞的自由基损伤:心肌细胞的自由基含量增高;动作电位及膜输入阻抗呈膜损伤性表现;超微结构呈现以肌丝、线粒体损伤为主的克山病样病理改变。向培养基中添加硒、锰,能对抗X-XOD所致的氧化性损伤。X-XOD was added into culture medium to induce superoxide anion free radical and initiate oxidative damage of rat myocardium. This model was used to observe tie protective effect of Se and Mn, taking action potential, input impedance, ESR spectrum and electron microscope appearance of cultured cells as indexes. Our results suggested that free radical damage induced by X-XOD can be antagonized by supplementation of Se and Mn into culture medium.

关 键 词：克山病 自由基 硒 锰 心肌细胞 

分 类 号：R542.302[医药卫生—心血管疾病]