薄荷不同组分单次给药对小鼠肝毒性“量-时-毒”关系比较研究  

Experimental Study on the "Dose-time-toxicity" Relationship of Hepatotoxicity Induced by Different Components from Herba Menthae in Mice

作　　者：李晓宇[1,2] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2012年第3期129-133,共5页Chinese Journal of Pharmacovigilance

基　　金：国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802);山东省科技平台建设项目课题(2008GG2NS02021)

摘　　要：目的观察薄荷挥发油与水提组分单次给药对小鼠肝毒性"量-时-毒"关系的影响。方法"时-毒"关系研究:取小鼠按不同时间点分组,单次灌胃给予一定剂量的薄荷挥发油和水提组分,观察给药后小鼠死亡情况和毒性反应,分别于药后不同时间检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST),计算肝、脾、胸腺脏器指数。"量-毒"关系研究:取小鼠按不同剂量分组,单次灌胃给予不同剂量的薄荷挥发油、水提组分分别于药后12 h、8 h按"时-毒"研究方法对小鼠进行相应处理。结果小鼠灌胃较高剂量薄荷挥发油后,血清ALT、AST活力在12 h达到高峰,持续时间约达48h;给药6 h后小鼠出现肝脏明显肿大,肝指数升高,其中6～24h肝脏指数升高较为明显。小鼠灌胃较高剂量薄荷水提组分后,血清ALT、AST活力在8h达到高峰,持续时间约达24h;给药4h后小鼠出现肝脏明显肿大,肝指数升高,其中4～12h肝脏指数升高较为明显。薄荷挥发油剂量在(0.2～0.5)mL.kg-1,水提组分剂量在(3.0～64.0)g.kg-1之间,对肝组织产生明显损伤,且随着剂量增大,ALT、AST升高显著。结论小鼠单次灌胃给予一定剂量的薄荷挥发油或水提组分可造成急性肝损伤,并呈现一定的"量-时-毒"关系。关于其肝脏损伤的机制有待进一步研究。Objective To study the ＂dose-time-toxicity＂ relationship of hepatotoxicity caused by single dose peppermint oil and water extract of Herba Menthae to mice.Methods Mice are grouped according to different time or dose points,to observe the death condition and toxicity of mice,detect ALT,AST in serum,calculate liver,spleen,thymus index.Results Compared with the control group,ALT,AST level in serum of water extracted components are peaked after 12 hours＇ administration,and restored near normality after 48 hours;ALT,AST level in serum of water extract components are peaked after 8 hours＇ administration,and AST level in serum of water extract components are peaked after 8 hours＇ administration,and restored near normality after 48 hours;The peppermint oil of（0.2~0.5） mL·kg-1 and water extract of（3.0~64.0） g·kg-1 have obvious damage to the liver organization,and ALT,AST inserum increased significantly with the dose increasing.Conclusion The peppermint oil and water extract gavaged certain dose one time to mice could cause obvious acute hepatotoxicity,and there were certain time-toxicity and dose-toxicity relationships.

关 键 词：薄荷 不同组分 小鼠 肝毒性 “量-时-毒”关系 

分 类 号：R285.5[医药卫生—中药学]