护心康联合静脉骨髓间质干细胞移植对急性心梗后大鼠心功能的影响  

The effect of Huxinkang combined with intravenous transplantation of MSCs on the cardiac function of AMI rats

作　　者：李新[1] 朱莹[1] 杨晖[1] 易健[2] 刘峥嵘[3] 谢勇[2] 

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005 [2]湖南中医药大学血管生物学实验室,湖南长沙410007 [3]湖南中医药大学湘杏学院

出　　处：《中医药导报》2012年第2期20-23,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省教育厅一般项目(10C1055);湖南省中医药大学青年基金

摘　　要：目的:研究复方中药对移植骨髓间充质干细胞(MSCs)在心肌梗死组织中的迁移、分化以及心肌梗死大鼠心功能的影响。方法:培养SD大鼠骨髓间充质干细胞,将各组大鼠分别用护心康和生理盐水灌胃7 d,第8天用改进的方法制作大鼠心肌梗死模型。在造模成功后第3天分别对MSCs移植组、护心康联合MSCs治疗组大鼠经尾静脉将0.5 mLMSCs悬液缓慢注射到相应大鼠体内(MSCs移植物中含1×107个干细胞),护心康组仍以护心康进行灌胃治疗。移植后,精心饲养3周后测定心功能,并行免疫组织化学检测。结果:MSCs移植组、护心康组、护心康联合MSCs治疗组移植3周后与术后1 d,心功能测定,EF对比:移植3周后,明显改善(P<0.001);FS对比:移植3周后,明显改善(P<0.001)。BrdU免疫组织化学染色显示MSCs移植组、护心康联合MSCs治疗组心脏标本切片可见有阳性染色的移植细胞存在,其余各组染色均为阴性。此外,BrdU免疫组化染色阳性细胞cTn-T染色也呈现阳性。结论:中药复方护心康能够促进经静脉移植骨髓间充质干细胞对梗死大鼠的心脏保护作用,增强其归巢的能力可能是机制之一。Objective：The purpose of this study was to research the effect of compound traditional chinese medicine on the migration and differentiation of bone marrow mesenchymal stem cells（MSCs） in the infracted areas of myocardial infarction ,and the change on left heart function of acute myocardial infarction（AMI）rats after the transplantation.Methods：SD rats were cultured bone marrow mesenchymal stem cells , The left anterior descending coronary artery was ligated to prepare the rat model with myocardial ischemia.sham-AMI group were only subjected to opening breast and traversing the thread without ligating coronary artery. MSCs transplantation group and HuXinKang combined MSCs group were given MSCs（lxl0v） through tail vein. All rats were killed and the hearts were obtained to perform immunohistochemical analysis after three weeks.Results： One day after surgery, except sham-AMI group,there were no dieffrences in FS and LVEF among other groups （P〉0.05）. Contrasting with one day after AMI,cardiac function（FS and LVEF） of group MSCs transplantation group, HuXinKang group and HuXinKang combined MSCs group presented significant improvements 3 weeks after cells transplantation （P〈0.001, respectively）. HE stain showed the scar fiber tissue in all specimens except sham-AMI rats. Only slides of group C and E displayed positive to staining with anti -- BrdU. In addition, anti-BrdU stain positive cells expressed Tropnin-T.Conclusion： Compound traditional chinese medicine HUXINKANG could improve the protection on the infracted areas by intravenous transplantation of MSCs. One of the mechanisms may be enhance the homing ability.

关 键 词：急性心肌梗死 骨髓间质干细胞 护心康 移植 静脉注射 

分 类 号：R285.5[医药卫生—中药学]