初情期前枫泾小母猪超数排卵及其胚胎发育研究(英文)  

STUDIES ON SUPEROVULATION AND EMBRYO DEVELOPMENT IN VIVO AFTER ARTIFICIAL FERTILIZATION OF FENGJING PUBERAL GILTS

作　　者：张德福[1] 王凯[1] 王英[1] 陈茵[2] 张平[1] 鲍世民[3] 王建荣[1] 

机构地区：[1]上海市农业科学院畜牧兽医研究所 [2]上海第二医科大学附属新华医院,上海200086 [3]中国科学院上海实验动物中心,上海200233

出　　处：《上海农业学报》2000年第1期38-41,共4页Acta Agriculturae Shanghai

基　　金：This study was supported by the Young Science Foundation of Shanghai Academy of Agricultural Sciencesl;NaturalScience &&

摘　　要：24头初情期前的枫泾小母猪以 PMSG、HCG进行超排处理 ,平均获得 2 3.4枚卵子 ,人工授精后 2 8～ 5 0 h超排的卵母细胞的受精率为 98.1% ( 472 /4 81) ,受精卵发育至桑椹胚和囊胚的比例为 89.7% ( 5 2 /5 8)。采用外科手术将 180枚受精卵移植至 8头同期发情的枫泾母猪子宫 ,其中有 5头妊娠 ,产仔 44头 ,胚胎移植妊娠率为 62 .5 %。Twenty four prepuberal Fengjing gilts were superovulated by using PMSG and HCG and on an average 23.4 eggs were collected per donor.At 28 to 50h after artificial insemination,the fertilization rates in vivo of oocytes from superovulated gilts in vitro were 98.1%(472/481).The developmental rates from fertilized eggs to morulae and blastocysts were 89.7%(52/58).A total of 180 fertilized eggs or 2 cell stage embryos were transferred surgically into the oviducts of estrus synchronized recipient gilts.Five of 8 recipients became pregnant,carried to term and produced 44 piglets.A pregnant rate of 62.5% resulted from 8 recipients after embryo transfer.

关 键 词：初情期前 小母猪 超数排卵 胚胎移植 胚胎发育 

分 类 号：S828.35[农业科学—畜牧学]