人参总皂苷对DC2.4细胞增殖、抗原吞噬和表面分子表达的影响  

Influence of total ginsenosides on proliferation,phagocytosis and expression of surface molecules of DC2.4 cells

作　　者：殷胜骏[1] 彭桂英[1] 顾立刚[1] 周英武[1] 韩涛[1] 万巧凤[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2012年第3期552-555,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金资助项目(No.30701105);北京中医药大学自主选题项目(No.2009JYBZZ-XS016)~~

摘　　要：目的:观察人参总皂苷对树突状细胞系DC2.4细胞增殖、抗原吞噬和表面分子表达的影响。方法:体外培养DC2.4细胞,加入不同剂量人参总皂苷后,MTT法检测不同时点细胞增殖;流式细胞仪检测细胞表面分子CD40、CD86和MHCⅡ的表达,以及对卵清蛋白抗原的吞噬作用。结果:单纯加入脂多糖(LPS)经培养48h后DC2.4细胞增殖明显(P<0.05),人参总皂苷可明显抑制LPS诱导的DC2.4细胞增殖(P<0.05);此外,人参总皂苷可促进DC2.4细胞吞噬抗原的能力,并协同LPS上调CD40、CD86和MHCⅡ分子表达。结论:人参总皂苷可以维持DC2.4细胞数量的稳态,促进DC2.4细胞吞噬抗原,并与LPS协同促进DC2.4细胞成熟,并具有促进抗原提呈的作用。Objective： This study is aimed at exploring the influence of total ginsenosides（TGs） on cell proliferation,phagocytosis and the expression of surface molecules of DC2.4 cells.Methods： DC2.4 cells were treated with different concentrations of TGs or/and LPS for the indicated times.Next cell proliferation studies were detected by MTT assay.The phagocytosis and expression of the surface molecules MHC-Ⅱ,CD40 and CD86 were detected by flow cytometry.Results： TGs could significantly revert the proliferation responses stimulated by LPS on DC2.4 cells after 48 hours of incubation（P〈0.05）.Besides,TGs obviously up-regulated the phagocytosis of immature DCs for FITC-OVA antigen as well as the expression of the surface molecules CD40,CD86 and MHC-Ⅱon DC2.4 cells stimulated by LPS.Conclusion： TGs maintained stable state of DC2.4 cells population,enhanced phagocytosis,and promoted maturation with LPS,which may facilitate antigen presentation of DC2.4 cells.

关 键 词：人参总皂苷 DC2.4细胞 细胞增殖 抗原吞噬 表面分子 

分 类 号：R285[医药卫生—中药学]