参元丹含药血清对缺氧复氧心肌细胞自噬的影响  

Influence of medicated serum with Shenyuan Dan on cardiomyocytes autophagy induced by hypoxia/reoxygenation

作　　者：解欣然[1] 张蕾[1] 尚菊菊[2] 林燕[1] 张璐[1] 刘红旭[2] 

机构地区：[1]北京市中医研究所,北京100010 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《中华中医药杂志》2012年第3期559-562,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30873246);北京市自然科学基金项目(No.7092042)~~

摘　　要：目的:观察参元丹含药血清对缺氧复氧心肌细胞自噬的影响,探讨参元丹心肌保护作用机制。方法:建立缺氧复氧心肌细胞损伤模型,采用免疫细胞化学法观察心肌细胞微管相关蛋白1轻链3(LC3)和磷酸肌醇-3-激酶(PI3K)表达,实时荧光定量PCR(Real-time PCR)法测定自噬相关基因(Beclin-1)和半胱天冬氨酸蛋白酶-3(Caspase-3)mRNA表达量。结果:与正常对照组相比,缺氧复氧组LC3和PI3K荧光表达增强,参元丹含药血清组与相应浓度空白血清组相比,细胞LC3和PI3K荧光表达强度减弱,其中参元丹含药血清10%组PI3K荧光强度明显降低,具有统计学意义(P<0.05);缺氧复氧组细胞Beclin-1和Caspase-3 mRNA表达量明显升高,与相应浓度空白血清组相比,参元丹含药血清组Beclin-1 mRNA表达水平明显降低;参元丹含药血清5%组Caspase-3 mRNA表达量降低。结论:参元丹含药血清对缺氧复氧细胞自噬具有抑制作用,可能与抑制PI3K,下调Beclin-1和Caspase-3基因有关。Objective： To study the influence of medicated serum with Shenyuan Dan（SYD） on autophagy of cardiac myocyte induced by hypoxia/reoxygenation（H/R）,and investigate its myocardial protective mechanism.Methods： To establish myocardial H/R injury model.The expressions of LC3 and PI3K in cardiac myocyte were observed respectively using immunocytochemistry.The mRNA expressions of Beclin-1 and Caspase-3 in cell were determined using real-time PCR.Results： Compared with control group,both the fluorescence expression of LC3 and PI3K in I/R group increased,while the expression of LC3 in medicated serum with SYD group decreased,and the expression of PI3K in 10% medicated serum with SYD group obviously decreased and showed statistically significance（P〈0.05）;The mRNA expression of Beclin-1 and Caspase-3 in I/R group increased.Medicated serum group,compared with control serum group,the mRNA expression of Beclin-1 decreased,and that of Caspase-3 in 5% group decreased.Conclusion： Medicated serum with SYD had inhibition on cardiomyocytes autophagy induced by H/R.The mechanism might be related to restrain PI3K,and down-regulate the gene of Beclin-1 and Caspase-3.

关 键 词：心肌细胞 自噬 缺氧复氧 参元丹 

分 类 号：R285[医药卫生—中药学]