逍遥散对慢性应激损伤大鼠NR受体亚型表达的影响  

Study on mechanism of Xiaoyaosan's regulation on feedback of NR in chronic stress rats

作　　者：富文俊[1] 敖海清[1] 孙琪[2] 徐志伟[1] 

机构地区：[1]广州中医药大学,广州510006 [2]宁夏医科大学,银川750004

出　　处：《中华中医药杂志》2012年第3期594-598,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30801525);广州中医药大学创新基金项目(No.K0100071);广东省普通高校重点实验室开放课题(No.AAF108111A29)~~

摘　　要：目的:本研究用逍遥散和糖皮质激素受体(GR)阻滞剂干预慢性应激损伤大鼠,观察其海马区NR受体亚型表达情况。方法:以逍遥散(5.265g/kg)和GR受体阻滞剂RU-38486(12.5g/kg)为干预药物,应用慢性不可预知应激对大鼠进行为期3周的造模,在造模的同时给予实验药物,3周后停止刺激,于第22天处死大鼠取材。采用荧光免疫组织化学法,测定各组大鼠海马区NR表达。结果:GR受体阻滞剂RU-38486与中药复方逍遥散可显著下调慢性应激损伤大鼠海马区NR阳性表达。结论:逍遥散可以通过促进慢性应激损伤大鼠海马区NR表达下调,从而达到部分恢复慢性应激大鼠下丘脑—垂体—肾上腺(HPA)轴负反馈功能的作用,起到缓解诸应激症状的疗效。Objective： We treat the chronic stress model by Xiaoyaosan and RU-38486,observing the expression of NR in brain hippocampus.Methods： We used chronic unpredicted stress method to establish the rat model for 3 weeks and gave the drugs including the Xiaoyaosan（5.265g/kg）and RU-38486（12.5g/kg） to intervene at the same time.On the 22nd day we observed the expression of NR by Fluorescent immunohistochemistry method.Results： Xiaoyaosan and RU-38486 could obviously down-regulated the the expression of intracellular NR subspecies in brain hippocampus of chronic stress rats.Conclusion： Xiaoyaosan may have the effect of relieving the stress symptoms by down-regulating the expression of intracellular NR subspecies in brain hippocampus and recovering the function of HPA of chronic stress rats.

关 键 词：逍遥散 慢性应激损伤 N-甲基-D-天门冬氨酸受体 

分 类 号：R285.5[医药卫生—中药学]