银花合剂对急性肺损伤大鼠血浆IL-1β含量与海马CA2-3区c-fos基因表达的影响  

Effect of Yinhua Agent on expression of c-fos proto-oncogene in the hippocampus and content of IL-1β after acute lung injury in CA2-3 regoin of rats

作　　者：姚丽君[1] 师晶丽[2] 吕明庄[3] 贺志光[3] 覃仁安[3] 

机构地区：[1]西南大学医院,重庆400715 [2]中山大学附属第五医院,珠海519000 [3]贵阳医学院,贵阳550004

出　　处：《中华中医药杂志》2012年第3期622-624,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探讨银花合剂对急性肺损伤(ALI)大鼠干预作用与可能机制。方法:采用油酸加内毒素"二次打击"方法复制大鼠ALI模型,用银花合剂进行干预,实验结束后测定动脉血氧分压、肺湿/干质量比值、肺病理形态学积分、血浆IL-1β的含量及大鼠海马CA2-3区FOS蛋白阳性细胞灰度值。结果:与对照组比较,模型组大鼠IL-1β含量显著升高(P<0.01),海马FOS阳性细胞灰度值迅速下降(P<0.01),与模型组相比,银花合剂组和地塞米松组氧分压上升,肺湿/干质量比值、病理形态学积分、IL-1β含量显著下降(P<0.01),FOS蛋白阳性细胞灰度值明显增高(P<0.01)。结论:银花合剂能够减轻肺损伤,其可能机制是银花合剂通过拮抗IL-1β,抑制海马c-fos表达实现对急性肺损伤大鼠的防治作用。Objective： To study the effects and possible mechanisms of Yinhua Agent on the acute lung injury（ALI）rats.Methods： To build the’Two hit’animal model of acute lung injure in rats induced with oleicacid and lipopolysaccharide.The levels of arterial partial pressure of oxygen,wet-to-dry weight ratio of lung,pathomorphological score,plasma IL-1β,gray value of fos positive cell in CA2-3 regoin of rats hippocampus were detected.Results： In comparison with normal group the level of plasma IL-1β tended to elevate significantly（P〈0.01）and the gray values of fos positive cell declined dramatically in model group（P〈0.01）.But the PaO2 was improved and the level of ratio W/D,pathomorphological score,plasma IL-1β were decreased significantly in Yinhua Agent and DEX groups compared with model group（P〈0.01）;the gray values in Yinhua Agent and DEX groups increased significantly（P〈0.01）.Conclusion： The results suggested that Yinhua Agent played a protective role in ALI.The mechanism of effects might be inhibition of the content of plasma IL-1β and the expression of hippocampus c-fos gene.

关 键 词：急性肺损伤 银花合剂 IL-1Β C-FOS 

分 类 号：R285.5[医药卫生—中药学]