电针对老年性痴呆大鼠记忆功能减退的影响  

Effect of electroacupuncture on memory hypofunction of Alzheimer' s Disease rats

作　　者：张晓琳 刘智艳 刘娟 郭慧 曲红岩 彭伟 鲍毅梅 殷丽莉 宋昳星 

机构地区：[1]新疆伊犁州新华医院康复理疗科,伊宁835000 [2]新疆维吾尔自治区中医医院针灸科,乌鲁木齐830000

出　　处：《中华中医药杂志》2012年第3期706-709,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：新疆维吾尔自治区自然科学基金项目(No.2009211A22)~~

摘　　要：目的:观察电针肾俞、风府、足三里、三阴交穴对老年性痴呆(AD)大鼠行为学和脑组织及血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量的影响。方法:筛选健康雄性Wistar大鼠30只,随机分为3组,假手术组、模型组、电针组,每组10只。其中模型组、电针组采用β淀粉样蛋白1-40(Aβ1-40)微量注射至大鼠右侧海马区建立AD模型,假手术组在相同位置注射同等剂量标准的生理盐水。模型组和假手术组不予以治疗,电针组治疗2个疗程后测试各组大鼠Y型电迷宫学习、记忆能力,检测脑组织及血清SOD、CAT活性及MDA含量,并进行统计分析。结果:假手术组、模型组治疗前后差异无统计学意义;治疗后电针组与模型组相比,差异有统计学意义(P<0.05)。结论:电针肾俞、风府、足三里、三阴交穴可以减少迷宫测试达标所需训练次数从而改善学习记忆功能,提高脑组织和血清SOD、CAT活性,降低MDA含量,其机制可能是电针提高了SOD、CAT抗氧化系统的活性增强了氧自由基清除能力而发挥了对神经元的保护作用。Objective： To observe effects of the behaviors of Alzheimer’s disease（AD） rats and the activity of superoxide dismutase（SOD）,catalase（CAT）,the content of malondialdehyde（MDA） in rats’ brain tissue and blood serum when apply electroacupuncture（EA） to the points of Shenshu,Fengfu,Zusanli,Sanyinjiao.Methods： To choose 30 healthy male Wistar rats,and divide them into fake surgical operation group,model group and EA group randomly,every group has 10 rats.Model group and EA group injected amyloid protein Aβ1-40 to rats’ right side hippocampus to build AD pattern.Fake surgical operation group injected the same dosage of saline in the same position.Model group and fake surgical operation group without any treatment.After two courses treatment,the ability of rats’ study and memory in the Y type labyrinth,the activity of SOD and CAT,and the content of MDA in rats’ brain tissue and blood serum were determined.Statistical analysis was used.Results： There was no statistically significant in model group and fake surgical operation group before and after treatment;There was statistically significant（P〈0.05） in EA group compared with model group after treatment.Conclusion： Applying EA to the points of Shenshu,Fengfu,Zusanli and Sanyinjiao,EA could reduce frequency of making AD rats through the test of Y type labyrinth,increase the activities of SOD and CAT,decrease the content of MDA in rats’ brain tissue and blood serum.The probably mechanism maybe that EA increased the activity of SOD and CAT’s antioxidation system to protect neurone,and strengthened the function of oxyradical’s purge system to improve antioxidation ability of body.

关 键 词：电针治疗 老年性痴呆 超氧化物歧化酶 过氧化氢酶 丙二醛 

分 类 号：R245[医药卫生—针灸推拿学]