粳籼89穗颈瘟分离菌株的致病力及遗传宗谱研究  

Studies on pathogenicity and its genetic structure of the blast isolates from Jingxian 89

作　　者：杨祁云[1] 朱小源[1] 伍尚忠[1] 张少红[2] 刘斌[2] 罗林[2] 

机构地区：[1]广东省农业科学院植物保护研究所,广州510640 [2]广东省农业科学院水稻研究所

出　　处：《植物保护》2000年第1期1-4,共4页Plant Protection

基　　金：广东省自然科学基金

摘　　要：通过对粳籼89的穗颈瘟分离菌株的致病力及遗传宗谱研究,结果表明:(1)粳籼89分离菌株的小种类型复杂,既有籼型小种如ZA1、ZB1、ZB5、ZB13、ZC13等,又有粳型小种如ZD5、ZG1等;(2)该类菌株的致病力存在不稳定性,同一菌株不同时间接种,鉴别寄主上表现的小种类型不同,对其他主栽水稻品种或抗源的致病力也表现不同,但这些菌株无论何时接种到粳籼89上均能使其表现感病,病级在4级以上;(3)该类菌株接种到粳籼89衍生品种或其他粳籼杂交后代上,一般能侵染这类品种;(4)利用RFLP技术,采用探针MGR586与限制性内切酶EcoRI组合对病菌进行DAN指纹分析,结果16个粳籼89分离菌株被分在2个相邻的遗传宗谱里,即宗谱1(9个菌株)和宗谱2(7个菌株),这两个宗谱恰好是广东的优势宗谱。Pathogenicity and its genetic structure of the blast isolates from Jingxian 89 were analyzed. The results indicated that reactions of the isolates on Chinese differentials were very different, indica races (ZA1,ZB1,ZB5,ZB13,ZC13) were identified in some isolates while japonica races (ZD5, ZG1) in others. The pathogenicity of the isolates to differentials and other resistant donor or cultivates appeared unstable, but appeared stable to jingxian 89 and its derives. The DNA fingerprinting analysis of 16 isolates from Jingxian 89 was conducted by RFLP, only two lineages, lineage 1 and lineage 2 which were belong to dominant lineages in Guangdong, were found among the tested isloates.

关 键 词：稻瘟病 致病力 毒力频率 遗传宗谱 抗病育种 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S511.034[农业科学—植物保护]