云南省种公猪精液中乙型脑炎病毒的监测  

Surveillance of Japanese encephalitis virus in boar semen in Yunnan Province

作　　者：李师师[1,2] 张文东[2] 吕粤[2] 赵焕云[2] 李昀海[2] 张武林[1,2] 岳亮[2] 张应国[2] 范泉水[3] 胡述光[1] 张富强[3] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]云南省动物疫病预防控制中心,云南昆明650051 [3]成都军区疾病预防控制中心,云南昆明650118

出　　处：《畜牧与兽医》2012年第2期1-4,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家公益性行业(农业)科研专项(201103008)

摘　　要：应用RT-PCR技术开展云南省种公猪精液中乙型脑炎病毒(JEV)感染监测,进而对阳性样品病毒基因扩增产物进行克隆、测序、比对及系统发育分析。从云南省16个地州797份猪精液中检出JEV阳性样品7份,阳性率0.88%。阳性精液样品中的JEV与基因Ⅰ型毒株PrM基因核苷酸序列同源性为97.5%～98.8%,与其他基因型毒株的同源性介于76.9%～89.8%之间,与疫苗毒株(基因Ⅲ型毒株,S19980008)的同源性为89.1%～89.8%。云南省种公猪精液中JEV属于基因Ⅰ型毒株,与人、猪、蚊虫基因Ⅰ型分离毒株遗传关系密切。The infection of Japanese encephalitis virus （JEV） in boar semen in Yunnan province was surveyed by RT-PCR. The JEV prm gene products from positive samples were cloned, sequenced and done alignment and phylogenetic analysis with known representative strains. 7 samples were demonstrated to be positive in 797 boar semen samples from 16 municipalities in Yunnan province. The positive rate was 0. 88%. The identities of nueleotide sequences of viral prm genes from the positive semen sample were 97.5% -98.8% with JEV genotype Ⅰ strains, 76.9% -89. 8% with other genotype strains, and 89. 1% -89.8% with the vaccine strain （ genotype Ⅲ） , respectively. The JEV isolates from boars in Yunnan province belonged to genotype Ⅰ and had close genetical relationship with JEV genotype Ⅰ strains isolated from human, swine and mosquito.

关 键 词：种公猪 精液 乙型脑炎病毒 监测 

分 类 号：S855.3[农业科学—临床兽医学]