Immobilization of laccase by Cu^(2+) chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol  

作　　者：Ying Wang Di Zhang Fu Rong He Xiao Chun Chen 

机构地区：[1]The Key Laboratory of Water and Sediment Sciences,Ministry of Education,School of Environment,Beijing Normal University,Beijing 100875,China [2]State Key Laboratory of Water Environment Simulation,School of Environment,Beijing Normal University,Beijing 100875,China [3]Department of Chemical Engineering,Beijing University of Chemical Technology,Beijing 100029,China

出　　处：《Chinese Chemical Letters》2012年第2期197-200,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos21177013 and 50708007);the National Basic Research Program of China(973 Project,No2010CB429003);the Fundamental Research Funds for the Central Universities;special fund of State Key Laboratory of Water Environment Simulation(No 11K04ESPCN)

摘　　要：Magnetic Cu^（2＋）-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination.The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol（PCP） degradation were evaluated systemically.Compared with free laccase,the immobilized laccase showed improved acid adaptabihty and thermal stability.The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.Magnetic Cu^（2＋）-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination.The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol（PCP） degradation were evaluated systemically.Compared with free laccase,the immobilized laccase showed improved acid adaptabihty and thermal stability.The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.

关 键 词：Magnetic silica Immobilization Laccase Pentachlorophenol 

分 类 号：O614.121[理学—无机化学] TS193.8[理学—化学]