三氯乙烯致敏豚鼠体内循环免疫复合物的变化  

Changes of circulating immune complexes in guinea pigs sensitized by trichloroethylene

作　　者：朱启星[1,2] 徐辉[1] 冷静[1] 沈彤[2] 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生系,安徽合肥230032 [2]安徽医科大学皮肤病研究所,安徽合肥230032

出　　处：《中国工业医学杂志》2012年第1期30-32,共3页Chinese Journal of Industrial Medicine

基　　金：国家自然科学基金资助项目(30872147);教育部科学技术研究重点项目(210100);安徽省自然科学基金项目(11040606M213);安徽高校省级自然科学研究重点项目(KJ2009A73)

摘　　要：目的检测循环免疫复合物(CIC)在三氯乙烯(TCE)致敏豚鼠体内含量的变化,探讨其可能的作用。方法选用体重250～300 g的白色雌性豚鼠,随机分成空白对照组、溶剂对照组、TCE处理组。根据豚鼠最大值试验(GPMT)方法处理豚鼠。分别在实验结束后24 h、72 h采血,检测血清中IgA/C3-CIC、IgG/C3-CIC和IgM/C3-CIC的浓度。结果 TCE处理组致敏率为65.38%;与溶剂对照组相比,TCE致敏组和TCE未致敏组的血清中IgA/C3-CIC、IgG/C3-CIC和IgM/C3-CIC的浓度均明显降低,差异有统计学意义(P<0.05)。结论 TCE处理组豚鼠血清中循环免疫复合物含量降低,可能提示其体液免疫发生改变。Objective To explore possible role of circulating immune complexes （CIC） played in guinea pigs （GPs） sen- sitized by trichloroethylene （TCE） and the related mechanism. Methods White female GPs （250 ~ 300 g） were randomly di- vided into blank control group, solvent （olive oil） control group and TCE treated group. GPs were treated according to Guinea Pig Maximization Test （GPMT）. 24 h and 72 h after administration, the blood samples were taken for detection of serum levels of IgA/C3-CIC, IgG/C3-CIC and IgM/C3-CIC with Kits. Results Sensitization rate of GPs by TCE was 65.38%, compared with those of solvent control group, the levels of IgA/C3-CIC, IgG/C3-CIC and IgM/C3-CIC in unsensitized and sensitized PGs by TCE were all significantly decreased （P 〈 0. 05 ）. Conclusion The study suggested that TCE treatment may affect the humor- al immunity in GPs.

关 键 词：三氯乙烯 豚鼠 循环免疫复合物 

分 类 号：R114[医药卫生—卫生毒理学] R623.221[医药卫生—公共卫生与预防医学]