三氯乙烯暴露致小鼠肝功能损害与Treg细胞的关系  

Relationship between trichloroethylene exposure induced liver damage and Treg cell in mice

作　　者：王进[1] 沈彤[1,2] 徐辉[1] 许述海[1] 江涛[1] 朱启星[1,2] 

机构地区：[1]安徽医科大学公共卫生学院,安徽合肥230032 [2]安徽医科大学皮肤病研究所,安徽合肥230032

出　　处：《中国工业医学杂志》2012年第1期33-35,共3页Chinese Journal of Industrial Medicine

基　　金：安徽省教育厅自然科学重点科研项目(KJ2009A73);安徽省自然科学基金项目(11040606M213);教育部科学技术研究重点项目(210100)

摘　　要：目的检测分析TCE暴露小鼠肝功能、肝脏Treg细胞分泌的细胞因子IL-10含量以及脾脏Treg细胞数量的变化,探讨Treg细胞与TCE暴露所致肝功能损害的关系。方法雌性BALB/c小鼠饮水摄入2.5 mg/ml和5 mg/ml的TCE,在2、4、8、12周时采集外周血用全自动生化仪测定肝功能,ELISA检测肝组织匀浆IL-10含量,流式细胞仪检测脾脏Treg细胞数量。结果与对照组相比,TCE染毒组小鼠AST和ALT水平明显升高,2周和4周时差异有统计学意义(P<0.05),肝组织匀浆中IL-10含量则明显降低(4周时最低);脾脏Treg细胞数量也明显降低,2、4、8周时差异均有统计学意义(P<0.05);相关分析显示肝功能与肝组织中IL-10含量和Treg细胞数量均呈负相关,且有统计学意义(P<0.05)。结论 TCE暴露引起的肝脏损伤与体内Treg细胞数量减少或功能受损相关。Objective To explore the relationships between Treg cell and trichloroethylene exposure induced liver damage through detecting liver function, IL-10 content secreted by hepatic Treg cell and number of spleen Treg cell in mice. Methods Female BALB/c mice were randomly devided into exposed groups that were given 2. 5 mg/ml or 5 mg/ml of TCE （ 10% DMSO solved） contained drinking water, blank group and solvent control group that were only given water or 10% DMSO. At the end of 2, 4, 8 or 12 weeks of exposure, take the blood samples for liver function examination, and the samples of liver and spleen were also taken for detecting IL-10 content in liver by ELISA, and Treg cell number in spleen by flow cytometry. Results Com- pared with control group, levels of AST and ALT in TCE treated groups were all significantly increased at 2 weeks and 4 weeks （ P 〈 0. 05 ）, liver IL-10 content decreased ; and spleen Treg cell number was also significantly decreased at 2,4,8 weeks （P 〈 0.05 ）. Correlation analysis showed that liver function negatively correlated with hepatic IL-10 level and number of spleen Treg cells （P 〈 0.05 ）. Conclusion These results suggested that liver damage induced by TCE exposure is well correlated to the func- tion of hepatic Treg cell and the number of spleen Treg cell.

关 键 词：三氯乙烯 肝脏毒性 TREG细胞 

分 类 号：R114[医药卫生—卫生毒理学] O623.221[医药卫生—公共卫生与预防医学]