外源钙对二氯喹啉酸胁迫下烤烟活性氧及保护酶的修复效应  

Repair Effects of Exogenous Calcium on Active Oxygen and the System of Protective Enzymes in Flue-cured Tobacco under Quinclorac Stress

作　　者：刘华山[1,2] 王晓军[1] 韩锦峰[1] 徐淑霞[2] 左涛[1] 张志勇[1] 袁仕豪 蒲文宣 易建华 

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002 [3]湖南中烟工业有限公司技术中心,湖南长沙410014

出　　处：《河南农业科学》2012年第2期64-67,共4页Journal of Henan Agricultural Sciences

基　　金：湖南中烟工业有限责任公司科技项目(2009160507)

摘　　要：研究了喷施不同浓度CaCl2溶液对二氯喹啉酸胁迫下烤烟活性氧和保护酶的修复效应。结果表明,同正常烟叶相比,二氯喹啉酸胁迫下的烤烟叶片超氧阴离子(O2.-)、羟自由基(.OH)、过氧化氢(H2O2)、丙二醛(MDA)及脯氨酸含量升高,而超氧化物歧化酶(SOD)及过氧化物酶(POD)活性下降;受害烟株喷施外源钙后,烟叶中O2.-、.OH、H2O2、MDA含量降低,SOD、POD活性提高,脯氨酸含量升高,与受害对照相比,喷施10mmol/L外源钙处理20d时,烟叶中O2.-、.OH、H2O2及MDA含量分别降低60%、49%、38%、42%;SOD、POD活性分别提高21%、81%;脯氨酸含量增加77%。喷施外源钙对二氯喹啉酸胁迫下烟叶生长有一定的修复作用,且喷施10mmol/L CaCl2时修复效果最佳。The study was designed to investigate,under quinclorac stress,repair effects of diffe-rent concentrations of exogenous calcium on active oxygen and the system of protective enzymes of flue-cured tobacco.It showed that,under quinclorac stress,the content of superoxide anion radi-cal（O-·2）,hydroxyl radical（·OH）,hydrogen peroxide（H2O2）,malondialdehyde（MDA） and proline increased,and the activity of superoxide dismutase（SOD） and peroxidase（POD） reduced.However,after spraying exogenous calcium,the indicators detected above tended towards a normal level except proline.After spraying 10 mmol/L CaCl2 for 20 days,the content of O-·2,·OH,H2O2 and MDA decreased 60%,48%,38% and 42%,respectively,and the activity of SOD,POD increased 21%,81%,respectively,as well as the content of proline increased 77%.Results suggested that spraying different concentrations of exogenous calcium had a positive repair effect on flue-cured tobacco under quinclorac stress,and the optimal concentration was 10 mmol/L.

关 键 词：烟草 除草剂 二氯喹啉酸 活性氧 保护酶 外源钙 

分 类 号：S572[农业科学—烟草工业]