深绿木霉SS003菌株抗病原真菌活性成分的基本性质  

Elementary Properties of Active Anti-fungal Compounds from Trichoderma atroviride Strain SS003

作　　者：敖新宇[1] 李永艳[2] 陈玉惠[1] 

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]西南林业大学林学院,云南昆明650224

出　　处：《河南农业科学》2012年第2期97-100,共4页Journal of Henan Agricultural Sciences

基　　金：西南林业大学重点基金项目(110706);云南省森林灾害预警与控制重点实验室资助项目(ZK09A105)

摘　　要：采用液体发酵和抑菌试验相结合的方法,研究了深绿木霉(Trichoderma atroviride)SS003菌株所产抗病原真菌类物质的基本性质。结果表明:(1)SS003发酵液对壳囊孢菌(Cytosporasp.)菌落生长的抑菌率为87.25%,发酵液中非蛋白等小分子部分的抑菌率为75.18%,而蛋白等大分子部分的抑菌率仅为51.03%;(2)不同极性的有机溶剂对SS003发酵液提取后,提取液对壳囊孢菌均具有一定的抑菌活性,抑菌率在28.95%～74.44%,其中甲醇提取液的抑菌率最高,达74.44%;(3)在0～121℃的温度范围内,抗菌活性成分(毒素)粗提液对壳囊孢菌的抑制活性没有发生明显变化,抑菌率在88.32%～95.81%;毒素粗提液在不同pH值条件下对壳囊孢菌菌落生长都有较强的抑制作用,抑菌率在81.25%～98.80%,其中在pH值为5.00～8.00时,毒素粗提液的抑菌效果最好,抑菌率均超过95%;(4)SS003菌株的毒素粗提液经活性炭吸附后,滤液对壳囊孢菌菌落生长有不同程度的抑制作用,其中抑制活性最强的滤液抑菌率达到60.31%。SS003菌株产生的抑制病原真菌壳囊孢菌菌落生长的活性成分种类复杂,性质多样,极性分布范围较广,具有一定的热稳定性和酸碱稳定性。The methods of liquid fermentation and anti-fungal test were adopted to study the elementary properties of anti-fungal compounds produced by Trichoderma atroviride strain SS003.The results were as follows：（1） The inhibition rate of SS003 fermentation liquor against the pathogenic fungus Cytospora sp.was 87.25%,and that of the non-protein micro-molecules was 75.18%,however,only 51.03% for the macro-molecules such as proteins.（2） The extracts from the fermentation liquor through different polar organic solvents had certain inhibition effects on Cytospora sp.（28.95%-74.44%）,in which the inhibition rate of the methanol extract was up to 74.44%,the highest of all.（3） In the temperature range of 0-121 ℃,the inhibition rate of active anti-fungal compounds（toxins） against Cytospora sp.was 88.32%-95.81%,not varying obviously with temperature.The toxins had strong inhibition effects on Cytospora sp.in the test pH range of 3.00-9.00 and the inhibition rate was 81.25%-98.80%.When the pH was within the limit of 5.00-8.00,the inhibition rate got over 95%.（4） After the toxin crude extract was adsorbed by activated carbon,the filtrates could inhibit the colony growth of Cytospora sp.to a certain degree,of which the strongest inhibitory rate was 60.31%.The active compounds of T.atroviride SS003 inhibiting Cytospora sp.were complex,with diversity and broad polar distribution as well as certain thermal and acid-base stability.

关 键 词：生防真菌 深绿木霉 活性成分 基本性质 抗真菌活性 病原真菌 壳囊孢菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]