维生素C对3种粪便隐血试验检测方法的影响  

Effect of vitamin C on the three test methods of fecal occult blood test

作　　者：梁骑[1] 贺巧[2] 刘畅[2] 王环[2] 王方建[2] 李君安[1] 吴昊[3] 唐中[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637100 [2]川北医学院检验系 [3]首都医科大学附属北京佑安医院感染科

出　　处：《中国实用医刊》2012年第5期6-8,共3页Chinese Journal of Practical Medicine

基　　金：国家自然科学基金（30872226）

摘　　要：目的探讨不同浓度维生素C（VitC）对3种粪便隐血试验（FOBT）检测方法的影响。方法以蒸馏水为对照，分别用联苯胺法、匹拉米洞法和胶体金法检测含有不同浓度VitC的粪便隐血阴性、阳性标本。结果体外试验显示，当VitC浓度分别为5、75、100、125、250mg／ml时，对3种方法检测FOBT阴性标本均不造成假阳性结果。VitC浓度为5、125、250mg／ml时，分别对联苯胺法、匹拉米洞法检测血红蛋白含量0．2—10、50～75、125mg／ml的阳性标本造成假阴性影响，且含血红蛋白量越多，VitC越不容易造成假阴性的影响；而对胶体金法无影响。结论体外实验显示不同浓度VitC，对联苯胺法、匹拉米洞法检测粪便隐血阴性标本不产生假阳性影响，对阳性标本会造成假阴性影响；对胶体金法无影响。Objective To investigate the effect of different contents of vitamin C on the three test methods of fecal occult blood test （FOBT）. Methods On the contrast of distilled water, Benzidine, Pyramidon, Colloidal gold were used to test samples with positive and negative result of FOBT with different contents of vitamin C. Results In vitro tests, there was no pseudo-positive result in negative samples of FOBT with 5, 75, 100, 125, 250 mg/ml of vitamin C by the three different test methods; however, when using positive samples of FOBT, pseUdo-negative results were found by the methods of Benzidine and Pyramidon when the contents of vitamin C were 5, 125, 250 mg/ml and the contents of hemoglobin were 0. 2 - 10, 50 -75, 125 mg/ml. What＇ s more, it was not so easy for the vitamin C to influence the positive sample when the hemoglobin becomes more and more, there was no effect on the method of colloidal gold. Conclusions In vitro tests, for the methods of Benzidine and Pyramidon, it has no in-fluence on negative samples of FOBT and it can result in pseudo-negative result when using positive samples of FOBT when the content of vitamin C is smaller than or equal to 250 mg/ml; For the method of Colloidal gold, it has no influence on negative and pos-itive samples of FOBT with different density of vitamin C.

关 键 词：维生素C 联苯胺法 匹拉米洞法 胶体金法 粪便隐血试验 

分 类 号：R446.1[医药卫生—诊断学]