微波加热法制备羧甲基壳聚糖-CdTe量子点及其在细胞标记中的应用  

Microwave-assisted aqueous synthesis of highly luminescent carboxymethyl chitosancoated CdTe/CdS quantum dots as fluorescent probe for live cell imaging

作　　者：何振宇[1,2] 朱洪浩[1] 周培疆[2] 

机构地区：[1]武汉市疾病预防控制中心,武汉430015 [2]武汉大学资源与环境科学学院

出　　处：《公共卫生与预防医学》2012年第1期4-7,共4页Journal of Public Health and Preventive Medicine

基　　金：863计划(2007AA06Z418);量子点标记细胞及生物大分子体系检测痕量环境有毒化学品毒性效应技术

摘　　要：目的快速制备壳聚糖-量子点荧光探针并将其用于细胞标记。方法优化前驱体溶液中的CdCl2、巯基乙酸、KHTe的比例,改变前驱体溶液的pH值和微波加热温度及时间,采用微波加热的方式在水溶液中快速合成CdTe量子点;为提高该CdTe量子点的生物相容性将其与羧甲基壳聚糖通过自组装,制备了壳聚糖-量子点荧光探针,用于狗肾细胞的标记。结果合成的壳聚糖-量子点荧光探针稳定时间超过10个月以上,荧光强度高,量子产率达到75%,半峰宽为42 nm,平均粒径约为3.5 nm,成功地用于了狗肾细胞标记。结论该荧光探针制备简便,荧光性质优异,具有较好的细胞相容性,在生物标记方面具有良好的应用前景。Objective To develope a simplified and rapid method for the synthesis of CMC-CdTe quantum dots（QDs） by microwave irradiation and using modified QDs for imaging live cells.Methods Several synthesis parameters,such as molar ratio of reagents,pH,reaction temperature,and reaction time were studied in details.Under the optimized conditions,highly effective CdTe QDs could be synthesized in aqueous phase.In order to improve the biocompatibility of the CdTe QDs,these QDs were then interacted with carboxymethyl chitosan（CMC） so as to be used as fluorescent probes for imaging live Madin-Darby canine kidney（MDCK）cells.Results With the incorporation of CMC,the stability of modified QDs was found to be improved significantly（from 4 months to more than 10 months at room temperature）.The photoluminescence quantum yield（PLQY） of the modified QDs could reach 75%,other specs include a full width at half maximum of the emission（FWHM） spectrum as 42 nm,and an average size,estimated from electron microscope graphing,as 3.5 nm.As fluorescent probes,these modified QDs were successfully used for imaging live MDCK cells.Conclusions The results indicated that these modified QDs demonstrated good biocompatibility and showed promising applications for bio-labeling and imaging.

关 键 词：CDTE量子点 羧甲基壳聚糖 荧光标记 

分 类 号：R12[医药卫生—环境卫生学] O657[医药卫生—公共卫生与预防医学]