四川凉山汉族2型糖尿病与脂联素基因多态性  

Association between single nucleotide polymorphism of adiponectin gene and type 2 diabetes in Han people in Liangshan,Sichuan province

作　　者：何慧婧[1] 卫大英[2] 王淳秀[1] 张建华[2] 黎晓彤[1] 马名驹[2] 王斌[1] 潘利[1] 单广良[1] 

机构地区：[1]中国医学科学院基础医学研究所流行病与卫生统计学系,北京100005 [2]四川省凉山彝族自治州疾病预防控制中心

出　　处：《中国公共卫生》2012年第3期302-304,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30671811)

摘　　要：目的探讨四川凉山彝族自治州汉族居民2型糖尿病与脂联素基因第2外显子第45位+45T/G单核苷酸多态性的关联及2型糖尿病危险因素。方法采用病例-对照研究方法,利用聚合酶链式反应—限制性片段长度多态性(PCR-RELP)技术,对2型糖尿病病例组与对照组进行SNP 45基因型检测,并测定身高、体重、血压、空腹血糖及血脂等指标。结果 SNP 45 TT、TG、GG基因型在病例组和对照组的频率分别为57.52%、35.29%、7.19%和55.70%、39.24%5、.06%;T,G等位基因在病例组和对照组的频率分别为75.16%、24.84%和75.32%2、4.68%,2组基因型频率与等位基因频率比较差异均无统计学意义;非条件logistic回归分析结果显示,体质指数和糖尿病家族史是凉山州汉族居民2型糖尿病的危险因素。结论脂联素基因SNP 45多态性与凉山州汉族居民2型糖尿病的关联尚不确定。Objective To assess the association between the single nucleotide polymorphism（SNP45） of adiponectin gene and type 2 diabetes and risk factors of type 2 diabetes in Han people in Liangshan Yi Autonomous Region,Sichuan province.Methods A case-control study was conducted and PCR-restriction fragment length polymorphism（RFLP） technique was used to analyze single nucleotide polymorphism（SNP45） among 153 cases and 158 controls,meanwhile physical and blood tests were carried out.Results The genotypes of TT,TG and GG of SNP45 accounted for 57.52%,35.29%,and 7.19% in the cases and 55.70%,39.24%,and 5.06% in the controls,respectively.The frequencies of T and G allele were 75.16% and 24.84% in the cases and 75.32% and 24.68% in the controls.There were no significant differences in the distribution of TT,TG,and GG genotype and T/G allele frequency of adiponectin gene between cases and controls.Multiple logistic regression analyses showed that body mass index（BMI） and family history of diabetes were associated with type 2 diabetes in Han people in Liangshan.Conclusion Association between SNP45 of adiponectin gene and type 2 diabetes is uncertain in Han people in Liangshan.

关 键 词：2型糖尿病 脂联素 单核苷酸多态性 病例对照研究 危险因素 

分 类 号：R181.3[医药卫生—流行病学]