血液透析及丙肝病毒感染者F蛋白抗体分析  

Prevalence of anti-HCV-F in hemodialysis patients with hepatitis C virus infection

作　　者：许可[1] 汤奋扬[1] 王昊鹏 王洁[3] 喻荣彬[3] 邓小昭[4] 张云[4] 

机构地区：[1]江苏省疾病预防控制中心急性传染病防制所,南京210009 [2]江苏省南京市卫生监督所 [3]南京医科大学公共卫生学院 [4]南京军区军事医学研究所

出　　处：《中国公共卫生》2012年第3期333-335,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81072343);江苏省自然科学基金(BK2011840)

摘　　要：目的探讨丙型肝炎病毒(hepatitis C virus,HCV)F蛋白抗体在血液透析人群HCV感染者中的分布特点及其影响因素。方法利用pEGX-4T-2/HCV-F融合载体表达蛋白HCV-F/GST作为抗原,包被酶联反应板,间接ELISA法检测128例血液透析HCV感染者血清中的HCV-F抗体;Logistic回归分析患者特征与HCV-F抗体是否阳性的关系。结果 128例HCV感染者中F抗体阳性率为45.3%;单因素分析显示,血透时间、HBcAb、HCV RNA与F抗体是否阳性的差异有统计学意义(分别P=0.038、P=0.012和P=0.002);多因素分析显示,血透时间>10年(OR=4.153,95%CI=1.435～12.023)、HCV RNA阳性(OR=3.6979,5%CI=1.498～9.123)是HCV-F抗体阳性的影响因素。结论血液透析人群HCV感染者中可检出F抗体;F蛋白的表达可能与HCV复制有关。Objective To assess the prevalence of hepatitis C virus F（HCV-F） antibody in hemodialysis patients with HCV infection.Methods The recombinant protein（HCV-F/GST） was coated onto micro titer plates as antigen.Sera of 128 renal dialysis patients with hepatitis C virus infection were tested by indirect enzyme-linked immunoabsorbent assay（ELISA） for detecting HCV-F antibody.Logistic regression was adopted to analyze the risk factors of anti HCV-F.Results Among the patients,58（45.3%） exhibited a positive anti-HCV-F reaction.Time of hemodialysis,co-infection with hepatitis B virus（HBV）,and HCV RNA were correlated with the expression of F protein（P=0.038,0.012,and 0.002）.Logistic regression identified that duration of hemodialysis ≥10 years（odds ratio=4.153,95% confidence intervall：1.435-12.023） and positive HCV RNA（OR=3.697,95%：1.498-9.123） were risk factors of the anti HCV-F.Conclusion Anti-HCV-F was detected in hemodialysis patients.Expression of F protein may be correlated with reproduction of HCV.

关 键 词：丙型肝炎病毒 F抗体 血液透析人群 

分 类 号：R183.9[医药卫生—流行病学]