黄颡鱼体表溃疡病病原菌分离鉴定及药敏试验  

Isolation and identification of pathogenic bacteria from Pelteobagrus fulvidraco ulcerative syndrome and its drug sensitive test

作　　者：黄钧[1] 温华成[1] 施金谷[1] 黄艳华[1] 梁正生 龙宜楠[1] 秦纪璇[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]永福县渔政渔港监督管理站,广西永福541800

出　　处：《南方农业学报》2012年第1期107-112,共6页Journal of Southern Agriculture

基　　金：广西水产畜牧兽医局专项项目(桂渔牧财[2009]88号;桂渔牧财[2010]58号)

摘　　要：【目的】对广西永福县某养殖场患溃疡病的黄颡鱼进行病原菌分离鉴定和药敏试验,为有效防治该病提供科学依据。【方法】用常规方法从患典型溃疡病的濒死黄颡鱼的心脏、肝脏及病灶等处取样分离病原菌,通过人工感染试验确定分离菌株的致病性,用API20NE生化鉴定系统对其进行鉴定,药敏试验采用纸片扩散法。【结果】分离获得的4株细菌(YFHS01、YFHS02、YFHS03和YFHS04)对健康黄颡鱼均有很强的致病性,都是黄颡鱼体表溃疡病病原菌,其中YFHS01为温和气单胞菌(Aeromonas sobria),YFHS02、YFHS03和YFHS04为嗜水气单胞菌(Aeromonas hydrophila)。4株病原菌对青霉素和氨苄青霉素均不敏感;对复达欣、先锋霉素VI、菌必治、庆大霉素、新霉素、氟哌酸、恩诺沙星、环丙沙星、多粘菌素B、左氟沙星、先锋必、特治星、氟苯尼考、盐酸沙拉沙星、复方磺胺嘧啶等15种药物高度敏感。【结论】广西永福县某养殖场黄颡鱼体表溃疡病是由嗜水气单胞菌与温和气单胞菌混合感染引起,可选用复达欣、先锋霉素VI、菌必治、庆大霉素、新霉素、氟哌酸、恩诺沙星、环丙沙星、多粘菌素B、左氟沙星、先锋必、特治星、氟苯尼考、盐酸沙拉沙星、复方磺胺嘧啶等15种药物进行防治。[Objective]The pathogenic bacteria were isolated and identified from ulcer diseased Pelteobagrus fulvidraco and drug sensitive tests were performed in order to develop effective control and prevention measures for disease.[Method]Pathogenic bacteria were extracted from the heart, liver and focus of nearly-dead Pelteobagrus fulvidraco, obtained from a farm in Yongfu County, Guangxi. Pathogenicity of the isolated strain was determined by artificial infection experiment. API 20NE biochemistry system was applied to identify pathogenic bacteria. Drug susceptibility test was conducted by using paper diffusion method. [Result]Four ulcer causing bacterial strains, viz., YFHS01, YFHS02, YFHS03 and YFHS04, found to possess strong pathogenicity to Pelteobagrus fulvidraco. The strain YFHS01 was Aeromonas sobria, while the rest were Aeromonas hydrophila. Four bacterial strains were insusceptible to penicillin and ampicillin, but highly susceptible to other 15 antibiotics, viz., ceftazidine, cephalosporinum, rocephin, gentamicin, neomycin, norfloxacin, enrofloxacin, ciprofloxacin, bacillosporin, levofloxacin, cefobid, tazocin, florfenicol, sarafloxacin hydrochloride and sulfadiazine. [Conclusion]The above mentioned antibiotics may be used to control and prevent ulcer disease in Pelteobagrus fulvidraco caused by Aeromonas hydrophila and Aeromonas sobria in a farm of Yongfu County, Guangxi.

关 键 词：黄颡鱼 溃疡病 嗜水气单胞菌 温和气单胞菌 分离鉴定 药敏试验 

分 类 号：S941.429[农业科学—水产养殖]