Highly Sensitive Functionalized Conducting Copolypyrrole Film for DNA Sensing and Protein-resistant  

作　　者：Zhang,Zhihong Li,Guijuan Yan,Fufeng Zhang,Zhonghou Fang,Shaoming 

机构地区：[1]Henan Provincial Key Laboratory of Surface&Interface Science,Zhengzhou University of Light Industry,Zhengzhou,Henan 450002,China

出　　处：《Chinese Journal of Chemistry》2012年第2期259-266,共8页中国化学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （No. 20704039）, Science ＆ Technology Innovation Talents in Universities of Henan Province （No. 2010HASTIT023） and International Science ＆ Technology Cooperation of Henan Province （No. 094300510078）.

摘　　要：In order to exploit the applications ofpolypyrrole （PPy） derivatives in biosensors and bioelectronics, the different immobilization mechanisms of biomolecules onto differently functionalized conducting PPy films are investigated. Pyrrole and pyrrole derivatives with carboxyl and amino groups were copolymerized with ω-（N-pyrrolyl）-octylthiol self-assembled on Au surface by the method of the chemical polymerization to form a layer of the copolymer film, i.e., poly[pyrrole-co-（N-pyrrolyl）-caproic acid] （poly（Py-co-PyCA）） and poly[pyrrole-co-（N-pyrrolyl）-hexylamine] （poly（Py-co-PyHA））, in which the carboxyl groups in poly（Py-co-PyCA） were activated to the ester groups. Based on the structure characteristics, the immobilization/hybridization of DNA molecules on PPy, poly（Py-co-PyCA） and poly（Py-co-PyHA） were surveyed by cyclic voltammograms measurements. For differently functionalized copolymers, the immobilization mechanisms of DNA are various. Besides the electrochemical properties of the composite electrodes of PPy and its copolymers being detected before and after bovine serum albumin （BSA） adsorption, the kinetic process of protein binding was determined by surface plasmon resonance of spectroscopy. Since few BSA molecules could anchor onto the PPy and its copolymers surfaces, it suggests this kind of conducting polymers can be applied as the protein-resistant material.In order to exploit the applications ofpolypyrrole （PPy） derivatives in biosensors and bioelectronics, the different immobilization mechanisms of biomolecules onto differently functionalized conducting PPy films are investigated. Pyrrole and pyrrole derivatives with carboxyl and amino groups were copolymerized with ω-（N-pyrrolyl）-octylthiol self-assembled on Au surface by the method of the chemical polymerization to form a layer of the copolymer film, i.e., poly[pyrrole-co-（N-pyrrolyl）-caproic acid] （poly（Py-co-PyCA）） and poly[pyrrole-co-（N-pyrrolyl）-hexylamine] （poly（Py-co-PyHA））, in which the carboxyl groups in poly（Py-co-PyCA） were activated to the ester groups. Based on the structure characteristics, the immobilization/hybridization of DNA molecules on PPy, poly（Py-co-PyCA） and poly（Py-co-PyHA） were surveyed by cyclic voltammograms measurements. For differently functionalized copolymers, the immobilization mechanisms of DNA are various. Besides the electrochemical properties of the composite electrodes of PPy and its copolymers being detected before and after bovine serum albumin （BSA） adsorption, the kinetic process of protein binding was determined by surface plasmon resonance of spectroscopy. Since few BSA molecules could anchor onto the PPy and its copolymers surfaces, it suggests this kind of conducting polymers can be applied as the protein-resistant material.

关 键 词：conducting polymer POLYPYRROLE cyclic voltammograms DNA immobilization protein adsorption 

分 类 号：O613.62[理学—无机化学] S432[理学—化学]