高羊茅对重金属镉的抗性分析  

Resistance Analysis to Cadmium in Tall Fescue(Festuca arundinacea)

作　　者：王晓桐[1] 柳玉霞[1] 苏旭东[1] 雷炳奇[1] 代亮[1] 徐吉臣[1] 

机构地区：[1]北京林业大学生物科学与技术学院/林木育种国家工程实验室,北京100083

出　　处：《中国农学通报》2012年第4期192-199,共8页Chinese Agricultural Science Bulletin

基　　金：北京自然科学基金项目"超积累植物镉富集关键基因的克隆和功能分析"(5122019)

摘　　要：为了解不同高羊茅品种对重金属镉的抗性差异,探讨其修复镉污染土壤的作用,通过形态和生理指标测定,对不同浓度镉胁迫前后的高羊茅品种形态和生理指标进行比较分析。结果表明:低浓度氯化镉胁迫下,高羊茅品种‘猎狗’的茎叶鲜重、最大根长、根鲜重、叶绿素含量等指标呈增长趋势,到达一定阈值后则受到抑制;相对电导率、MDA含量、脯氨酸含量则随镉浓度的增大而持续升高;根系活力则表现为随镉浓度的升高而逐渐下降。进一步利用50mg/L的氯化镉胁迫处理9个高羊茅品种,对茎叶鲜重、根鲜重、最大根长等形态指标的变化情况进行分析评估。试验结果显示,多数品种的抗性与根部性状的表现更为密切;综合抗性由强到弱的排列顺序为:‘交战Ⅱ’>'毕加索’>'猎狗’>'杰作’>‘新秀’>‘碧翠A’>‘可奇思’>‘爱瑞’>'达芬奇’。对于镉污染程度较高的土壤,可选择综合抗性较高的品种‘交战II’进行治理修复;对于镉污染程度较低的土壤,可选用抗性一般但地上部分生物量较高的品种‘新秀’。For understanding the resistance differences of tall fescue to cadimum pressure and how to work in recovering the cadmium contaminated soil, the author analyzed the variation of morphology and physiology index before and after the cadmium treatments in different concentrations. The author analyzed the variety Houndog with the treatment of different concentration of cadmium chloride. The results showed the leaf fresh weight, root fresh weight, maximum root length, and chlorophyll content stimulated in lower concentration of cadmium, but suppressed over the threshold value. The relative leakage ratio, MDA content, and proline content displayed continued increasing, while root activity declined with the concentration of cadmium increased. Further analysis of 9 tall fescue varieties was conducted for the traits of leaf fresh weight, root fresh weight, and maximum root length with the treatment of 50 mg/L cadmium chloride. The results showed the resistance mostly correlated with the performance of root characteristics. The resistance level totally ranked as Cross Fine Ⅱ〉Picasso〉Houndog〉Masterpiece〉Starlet〉TripleA〉CochiseⅢ〉Airui〉Davinci. Ⅱ concluded that the variety of Cross Fine Ⅱ with higher comprehensive resistance level to cadmium could be used in heavy contaminated soil, while the variety of Starlet could be screened in the minor contaminated soil.

关 键 词：高羊茅 镉 抗性 生理指标 表型指标 

分 类 号：Q89[生物学]