中国局部地区草坪立枯丝核菌的rDNA ITS序列分析  

Sequences Analysis of rDNA Internal Transcribed Spacer(ITS) of Rhizoctonia solani Kühn from Some Local Areas in China

作　　者：淮稳霞[1] 程衬衬[1] 李东林 赵文霞[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所/国家林业局森林保护重点实验室,北京100091 [2]北京新自然高尔夫球场建设有限公司,北京100102

出　　处：《中国农学通报》2012年第4期200-205,共6页Chinese Agricultural Science Bulletin

基　　金："十一五"国家科技支撑计划"草坪褐斑病快速诊断及控制技术"(2006BAD08A1003D);国家林业局948项目"栎树疫霉猝死病菌等森林疫霉检测鉴定技术及风险预警系统的引进"(2009-4-35)

摘　　要：为了鉴定草坪褐斑立枯丝核病菌的融合群类型并探讨其系统发育关系,对11株分离自草坪草和4株分离自土壤的立枯丝核菌(Rhizoctonia solani)进行了rDNA-ITS测序,结合Gen Bank中登录的立枯丝核菌14融合群或亚群的代表性菌株及禾谷丝核菌(R.cerealis)、玉蜀黍丝核菌(R.zeae)和水稻丝核菌(R.oryzae)3个近缘种,共45条ITS序列进行了分析,利用MEGA4.1软件建立ITS聚类分析树状图。结果表明,立枯丝核菌各融合群代表菌株及所有供试菌株聚为一组;该组又可细分为14个遗传聚亚类,隶属同一融合群的代表菌株聚在同一亚组,供试15株立枯丝核菌归入AG2-2和AG1-IA2个融合群。这些结果为该病害的科学防控提供了可靠依据,并进一步验证了ITS序列分析法是区分立枯丝核菌不同融合群或亚融合群菌株的有力工具。The internal transcribed spacer （ITS） region of the ribosomal DNA from 11 isolates of Rhizoctonia solani Ktihn from turfgrass and 4 from soil were sequenced in order to identify anastomosis groups and discuss phylogenetic relationships between them. Based on the 15 sequences and 30 published sequences of R. solani from 14 anastomosis groups or sub-groups, R. cerealis, R. zeae and R. oryzae in GenBank database, the ITS phylogenetic tree were generated with MEGA 4.1. The result showed that, all the representative isolates of known anastomosis groups and 15 samples tested were clustered into one group. In this group, representatives of the different anastomosis groups clustered separately, and 15 isolates sequenced were divided into AG2-2 and AGI-IA. These results provided a reliable basis for disease control, and also suggested that the sequence analysis of ITS rDNA region was a valuable tool for identifying anastomosis groups of R. solani.

关 键 词：草坪褐斑病 立枯丝核菌 菌丝融合群 RDNA-ITS 

分 类 号：S763.15[农业科学—森林保护学]