血清11型鸭疫里默氏菌的分离鉴定  

Isolation and Identification of Serotype 11 Riemerella Anatipestifer

作　　者：杨晓辉[1,2] 程龙飞[2,3] 陈红梅[2,3] 施少华[2,3] 傅光华[2,3] 万春和[2,3] 黄瑜[2,3] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]江西农业大学动物科学学院,南昌330045 [3]福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《中国农学通报》2012年第5期23-27,共5页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系建设专项资金"鸭疫里默氏菌侵染机制及功能基因解析"(CARS-43-07B);福建省公益类科研院所专项"鸭疫里默氏菌和鸭大肠杆菌二联灭活苗的研制"(2011R1025-6)

摘　　要：从同一发病鸭场间隔4天分离到两株鸭疫里默氏菌(RA1156和RA1157),为比较它们的差异。采用平板凝集试验和琼扩试验鉴定血清型,纸片法测定耐药性,动物试验测定致病性。结果显示:两株鸭疫里默氏菌的血清型相同,均为11型;对樱桃谷鸭的致病性相同;对大多数药物的敏感性相同,RA1156对庆大霉素和青霉素敏感,而RA1157对庆大霉素和青霉素耐药。结果确认了11型鸭疫里默氏菌在福建的存在,增添了福建省鸭疫里默氏菌的流行病学资料;与实验室人工诱导相比,体内的鸭疫里默氏菌更容易产生耐药性。Two strains of Riemerella anatipestifer （RAl156 and RA1157） had been isolated from the same duck farm interval of four days. To compare the differences of the two strains, plate agglutination and agar gel precipitation tests were used to identify the serotypes of them, KirbyBauer method was used to detect the drug sensitivities, and animal testing was used to determine the pathogenic of them. The results showed that both of two strains were serotype 11, had the same pathogenic to duck, and had the same sensitivities to most drugs. Strain RAl156 was sensitive to Gentamicin and Penicillin, while strain RAl157 was resistant to these two drugs. This test confirmed the presence of serotype 11 Riemerella anatipestifer in Fujian province and added the epidemiological data of Riemerella anatipestifer. Compared with laboratory artificially induced, the strain in vivo might get the resistance to drug easier.

关 键 词：鸭疫里默氏菌 分离鉴定 血清型 平板凝集试验 试验测定 庆大霉素 耐药性 致病性 

分 类 号：S852.61[农业科学—基础兽医学]