血液提取总RNA的初步研究  

Primary Study on RNA Extraction in Blood

作　　者：陈志[1] 罗卫星[1] 刘若余[1] 石照应[1] 王禄超[1] 张依裕[1] 蔡惠芬[1] 

机构地区：[1]贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵阳550025

出　　处：《中国农学通报》2012年第5期28-31,共4页Chinese Agricultural Science Bulletin

基　　金：贵州省农业科技攻关项目"利用分子育种技术选育黔北麻羊"[黔科合NY字(2008)3044]

摘　　要：试验兔血为材料,使用传统的TRIzol法和试剂盒提取总RNA。并用紫外分光光度仪分别测定样品在260、320、230、280nm的光度及估算RNA的纯度和浓度。结果表明:TRIzol法和试剂盒法相比较,试剂盒提取的RNA样品质量较高,而且简单快速。Taking the rabbit blood as the material, which were extracted total RNA by the traditional method of TRLzol and the RNA Extraction Kit. It had measured 260, 320, 230, 280 nm of sample luminosity by uvspectrophotometry, and estimated the purity and concentration of RNA. The results indicated that compared to the method of TRIzol, the RNA Extraction Kit was more high quality ,simple and fast.

关 键 词：兔 血液 总RNA提取 

分 类 号：Q38[生物学—遗传学]