日本血吸虫反式剪接前导RNA的鉴定  

Identification and Characterization of an mRNA Trans-splicing Leader in Schistosoma Japonicum

作　　者：李锐[1] 贺亮[2] 郝力力[3,4] 

机构地区：[1]浙江省农业科学院农产品质量标准研究所,杭州310021 [2]上海医药工业研究院新药安全评价中心 [3]西南民族大学 [4]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《中国农学通报》2012年第5期36-40,共5页Chinese Agricultural Science Bulletin

基　　金：中国医学科学院病原生物学研究所资助项目(2008IPB114)

摘　　要：RNA反式剪接现象广泛存在于真核生物中,包括单细胞原虫以及低等脊索动物。为鉴定日本血吸虫中是否存在SLRNA介导的反式剪接,运用Race方法从成虫中克隆出了1个90nt的SLRNA基因,36nt的RNA前导序列正是来源于此90nt的无PolyA结构的SLRNA,并通过Northern进一步证实了该基因的存在。同时采用荧光定量和Southern对其拷贝数、基因组上的分布方式以及虫体不同阶段的表达量进行了鉴定,发现SLRNA具有55个拷贝并在基因组上呈散在分布;在虫卵和尾蚴时期SLRNA基因的转录丰度最高,雌虫阶段最低,雄虫、3天童虫以及14天童虫阶段无明显差别。结果表明,SLRNA介导的反式剪接可能是日本血吸虫基因转录后重要的调控机制之一。RNA transsplicing occurs in a wide range of eukaryotes, from protozoa to chordates. To investigate the SL RNAmediated transsplicing in the zoonotic trematode Schistosoma japonicura, a splicedleader （SL） RNA was cloned in adults using race. The 36nucleotide SL in S. japonicum was derived from a 90nucleotide nonpolyadenylylated RNA transcript encoded by 55 copies of SL genes dispersed in the genome confirmed by blot and real time PCR. Differential transcription patterns of the SL gene in the parasite developmental stages were observed using real time PCR. Predominant expression of SL RNA was found in the two developmental stages, egg and cercariae. Expression of SL RNA transcripts in female was the least. No significant expression difference between male and schistosomulum （3 d and 14 d） was observed. In summary, transsplicing of spliced leader might acted as an important mechanism of posttranscriptional gene regulation in S. japonicum.

关 键 词：日本血吸虫 反式剪接 SLRNA 

分 类 号：Q78[生物学—分子生物学]