腺苷在小鼠脑组织中的分布  

Distribution of adenosine in the brain of mice

作　　者：陶芳[1,2] 马张庆[1,2] 洪宗元[1,2] 

机构地区：[1]皖南医学院定量药理研究所,安徽芜湖241002 [2]皖南医学院药理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2012年第1期1-3,共3页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(81171255);教育部科学技术研究重点项目(208058);安徽省自然科学基金项目(070413139)

摘　　要：目的:监测小鼠脑组织中的腺苷含量,探讨腺苷在各脑区中的分布。方法:雄性昆明种小鼠8只,在乙醚麻醉下断头处死,迅速取出脑组织并分离出皮层、海马、纹状体、丘脑、下丘脑、中脑等脑区。这些脑组织被制成匀浆,采用高效液相色谱-紫外(HPLC-UV)检测法检测其腺苷含量。结果:腺苷在小鼠纹状体、皮层及海马中含量较高,下丘脑和丘脑次之,中脑最低。结论:腺苷含量在小鼠各脑区中呈不均匀分布,其中纹状体内含量最高。Objective： To detect adenosine contents in brain homogenates for investigating the distribution of adenosine in the brain of mice.Methods： Eight male mice of Kunming strain were killed by decapitation under anesthesia by diethyl ether,and the brain tissues were quickly removed out and divided on ice into seven regions： cortex,striatum,hippocampus,hypothalamus,thalamus and midbrain.These brain tissues were homogenized and determined for the adenosine content by HPLC with ultraviolet detector.Results： The adenosine level was the highest in striatum,cortex and hippocampus,moderately downward for hypothalamus and thalamus,and the lowest in midbrain of the mouse brain.Conclusion： Adenosine content is unevenly distributed in different cerebral regions of mice in which the striatum holds maximally.

关 键 词：小鼠 腺苷 脑组织匀浆 高效液相色谱 

分 类 号：R963[医药卫生—微生物与生化药学]