高效液相色谱(HPLC)技术检测大豆异黄酮含量  

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF ISOFLAVONE CONTENT IN SOYBEAN TEST SAMPLES

作　　者：孙君明[1] 丁安林[1] 东惠茹[2] 

机构地区：[1]中国农业科学院作物育种栽培研究所,北京100081 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《大豆科学》2000年第1期15-20,共6页Soybean Science

摘　　要：利用高效液相色谱 (HPL C)技术分离并测定了大豆中异黄酮各组分含量 ,建立了快速、有效的分离、检测异黄酮含量的方法。结果显示 ,异黄酮的最适提取时间是2 - 3hr;最适检测波长为 2 6 0 nm;在一定的浓度范围内 ,标准样品 Daidzin和 Genistin作为异黄酮的检测标样是准确的 ;提取和检测温度对异黄酮组合含量有很大影响 ,在80℃或更高的温度条件下 ,丙二酰基结合体可水解为相应的甙或甙元的形式。Soybean isoflavone compounds were separated and determined with HPLC, and a simple sensitive and highly reproducible method was established. The result indicated the most suitable extracting time of isoflavine is 2-3hr, the suitable absorbent wavelength is 260nm; It is accurate for the isoflavone standard sample with daidzin and genistin at the range of concentration; The isoflavone content is affected by extracting and examining temperatures. At 80℃ or higher temperature, isoflavone malonyl conjugates are hydrolyzed to glucosides and aglycones.

关 键 词：大豆 高效液相色谱 异黄酮 分离 检测 

分 类 号：S565.1[农业科学—作物学]