An Investigation of Oxidative DNA Damage in Pharmacy Technicians Exposed to Antineoplastic Drugs in Two Chinese Hospitals Using The Urinary 8-OHdG Assay  

作　　者：HUANG Yu Wen JIAN Le ZHANG Mei Bian ZHOU Quan YAN Xiao Feng HUA Xu Dong ZHOU Ying HE Ji Liang 

机构地区：[1]The Second Affiliated Hospital of College of Medicine of Zhejiang University, Hangzhou 310009, Zhejiang, China [2]School of Public Health, Curtin Health Innovation Research Institute, Curtin University of Technology, Perth 6845, Australia [3]Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang, China [4]Sir Run Run Shaw Hospital of College of Medicine of Zhejiang University, Hangzhou 310020, Zhejiang, China [5]Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310012, Zhejiang, China [6]Zhejiang University, Medical College, Institute of Environmental Medicine, Hangzhou 310058, Zhejiang, China

出　　处：《Biomedical and Environmental Sciences》2012年第1期109-116,共8页生物医学与环境科学（英文版）

基　　金：supported by the Scientific Research Fund of Health Bureau in Zhejiang Province (2009A089);Scientific Research Fund of Education Bureau in Zhejiang Province (Y200804934)

摘　　要：Objective To investigate oxidative DNA damage in pharmacy technicians preparing antineoplastic drugs at the PIVAS （Pharmacy Intravenous Admixture Service） in two Chinese hospitals. Methods Urinary 8-OHdG served as a biomarker. 5-Fluorouracil （5-FU） concentrations in air, masks and gloves were determined. The spill exposure of each PIVAS technician to antineoplastic drugs was investigated. Eighty subjects were divided into exposed group t, II, and control group I, II. Results 5-FU concentration ratios for gloves and masks in exposed group I were significantly higher than those in exposed group II （P〈0.05 or P〈0.01）. The average urinary 8-OHdG concentrations in exposed group I, control group I, exposed group II, and control group II were 24.69＋0.93, 20.68＋1.07, 20.57＋0.55, and 12.96_＋0.73 ng/mg Cr, respectively. Urinary 8-OHdG concentration in exposed group I was significantly higher than that in control group I or that in exposed group 11 （P〈0.02）. There was a significant correlation between urinary 8-OHdG concentrations and spill frequencies per technician （P〈0.01）. Conclusion There was detectable oxidative DNA damage in PIVAS technicians exposed to antineoplastic drugs. This oxidative DNA damage may be associated with their spill exposure experience and contamination of their personal protective equipment.Objective To investigate oxidative DNA damage in pharmacy technicians preparing antineoplastic drugs at the PIVAS （Pharmacy Intravenous Admixture Service） in two Chinese hospitals. Methods Urinary 8-OHdG served as a biomarker. 5-Fluorouracil （5-FU） concentrations in air, masks and gloves were determined. The spill exposure of each PIVAS technician to antineoplastic drugs was investigated. Eighty subjects were divided into exposed group t, II, and control group I, II. Results 5-FU concentration ratios for gloves and masks in exposed group I were significantly higher than those in exposed group II （P〈0.05 or P〈0.01）. The average urinary 8-OHdG concentrations in exposed group I, control group I, exposed group II, and control group II were 24.69＋0.93, 20.68＋1.07, 20.57＋0.55, and 12.96_＋0.73 ng/mg Cr, respectively. Urinary 8-OHdG concentration in exposed group I was significantly higher than that in control group I or that in exposed group 11 （P〈0.02）. There was a significant correlation between urinary 8-OHdG concentrations and spill frequencies per technician （P〈0.01）. Conclusion There was detectable oxidative DNA damage in PIVAS technicians exposed to antineoplastic drugs. This oxidative DNA damage may be associated with their spill exposure experience and contamination of their personal protective equipment.

关 键 词：Urinary 8-OHdG Oxidative DNA damage Antineoplastic drugs Occupational exposure Pharmacy Intravenous Admixture Service 

分 类 号：TQ464.7[化学工程—制药化工] Q523[生物学—生物化学]