Promoter Activities of Genes cpcT2 and cpcS2 in Nostoc PCC 7120  

作　　者：CHEN Yu CHEN Sili LIU Li SUN Yafang ZHOU Ming ZHAO Kaihong ZHANG Juan 

机构地区：[1]College of Environment Science and Engineering, HuazhongUniversity of Science and Technology, Wuhan 430074, Hubei,China [2]College of Life Science and Technology, South-CentralUniversity for Nationalities, Wuhan 430074, Hubei, China [3]State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University, Wuhan 430070, Hubei, China

出　　处：《Wuhan University Journal of Natural Sciences》2012年第2期169-176,共8页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China(30870541, 31070743);China Postdoctoral Science Foundation (20100471191)

摘　　要：To study the promoter activities of genes cpcT 2 and cpcS 2,several upstream DNA fragments of these two genes with different lengths were selected.These fragments were fused with promoterless gfp（reporter gene） for constructing five recombinant plasmids.Then,these recombinant plasmids were transferred into Nostoc PCC 7120 by conjugation.The promoter activities of genes cpcT 2 and cpcS 2 were determined by observing the fluorescence of green fluorescent protein（GFP） with a fluorescence microscope.The result showed that the 1 300 bp（-1 300 to 0 bp） and 2 600 bp（-2 600 to 0 bp） upstream fragments of cpcT 2 had strong promoter activity and the promoter activity of the 680 bp（-680 to 0 bp） fragment was weaker than that of two above fragments of cpcT 2.The 2 000 bp（-2 000 to 0 bp） upstream fragment of cpcS 2 revealed weak promoter activity.This showed that a strong promoter of cpcT 2 was located in the upstream fragment between-680 and-1 300 bp.To study the promoter activities of genes cpcT 2 and cpcS 2,several upstream DNA fragments of these two genes with different lengths were selected.These fragments were fused with promoterless gfp（reporter gene） for constructing five recombinant plasmids.Then,these recombinant plasmids were transferred into Nostoc PCC 7120 by conjugation.The promoter activities of genes cpcT 2 and cpcS 2 were determined by observing the fluorescence of green fluorescent protein（GFP） with a fluorescence microscope.The result showed that the 1 300 bp（-1 300 to 0 bp） and 2 600 bp（-2 600 to 0 bp） upstream fragments of cpcT 2 had strong promoter activity and the promoter activity of the 680 bp（-680 to 0 bp） fragment was weaker than that of two above fragments of cpcT 2.The 2 000 bp（-2 000 to 0 bp） upstream fragment of cpcS 2 revealed weak promoter activity.This showed that a strong promoter of cpcT 2 was located in the upstream fragment between-680 and-1 300 bp.

关 键 词：Nostoc PCC 7120 promoter cpcT 2 cpcS 2 green fluorescent protein（GFP） 

分 类 号：Q789[生物学—分子生物学]