HPLC法测定清喉咽颗粒中连翘苷的含量  

Determination of Phillyrin in Qinghouyan Granules by HPLC

作　　者：史大军 邱海蕴 康红英 

机构地区：[1]湖北省宜昌市食品药品监督检验所,宜昌443003

出　　处：《中国药事》2012年第1期57-58,71,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立高效液相色谱法测定清喉咽颗粒中连翘苷的含量。方法采用Diamonsil C18色谱柱,以甲醇-水(25∶75)为流动相,流速为1.0mL.min-1,检测波长为277nm。结果线性范围为0.0496～0.2976μg,平均回收率为99.2%,RSD为0.7%(n=9)。结论本法简便、准确、重复性好,可用于该制剂质量控制。Objective To develop an HPLC method for determining the content of phillyrin in Qinghouyan Granules.Methods A diamonsil C18 column was adopted.The mobile phase was methanol-water（25∶75） with flow rate of 1.0mL·min-1.The detection wave length was set at 277nm.Results Good linearity was obtained from 0.0496 to 0.2976μg for phillyrin;the mean recovery（n=9） was 99.2%.and RSD was 0.7%.Conclusion The method is simple and accurate with good reproducibility.It can be applied to the quality control of Qinghouyan Granules.

关 键 词：高效液相色谱法 连翘苷 清喉咽颗粒 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]