HPLC法测定双黄连颗粒中绿原酸的含量  

Determination of Chlorogenic Acid in Shuanghuanglian Granules by HPLC

作　　者：赵霞[1] 杨丽[2] 

机构地区：[1]云南省保山市食品药品检验所,保山678000 [2]大理学院药学院

出　　处：《中国药事》2012年第1期59-60,63,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立双黄连颗粒中绿原酸含量测定的HPLC分析方法。方法采用ZORBAX SB-C18色谱柱,流动相为乙腈-0.4%磷酸(13∶87),检测波长为330nm,流速为1.0mL.min-1。结果绿原酸在0.0662～0.6620μg范围内线性关系良好,r=0.99963,平均回收率为97.6%,RSD为0.96%(n=9)。结论本方法操作简便、准确、重复性好,可用于双黄连颗粒中绿原酸的含量测定。Objective To establish a method for determination of chlorogenic acid in Shuanghuanglian Granules by HPLC.Methods The quantitative analysis was performed on a ZORBAX SB-C18 column.The mobile phase was acetonitrile-0.4% phosphoric（13∶87）with the flow rate of 1.0mL·min-1.The detection wavelength was at 330nm.Results The linear range of chlorogenic acid was 0.0662~0.6620μg（r=0.99963）.The average recovery was 97.6%,and RSD was 0.96%（n=9）.Conclusion The method is simple,accurate,and can be used for the quality control of this preparation.

关 键 词：双黄连颗粒 绿原酸 HPLC 含量测定 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]