稀土元素铕在南丰蜜橘园能流定量研究中的应用  

Quantitative Research on the Energy Flow of Ecosystem in Nanfeng Orange Park by Applying Rare Earth Element Europium

作　　者：袁凤辉[1] 陈连水[1] 饶军[1] 白涛[1] 

机构地区：[1]东华理工大学生物系,江西抚州344000

出　　处：《安徽农业科学》2012年第7期3949-3952,共4页Journal of Anhui Agricultural Sciences

基　　金：江西省教育厅科技项目(GJJ10495)

摘　　要：[目的]深入了解南丰蜜橘、害虫、天敌间的3级营养关系以及定量描述害虫与天敌的种群动态,以有效推动南丰蜜橘园生态控虫技术的发展。[方法]利用改良的稀土元素铕荧光物示踪法和食物链传递标记定量分析法研究南丰蜜橘园中橘叶-害虫-天敌食物链间的能量流动,并利用灰化、显色、紫外测定和吸光度换算等生物化学方法测定各营养级的铕含量;将各营养级之间的能流关系进行量化,从而确定自然状态下害虫对南丰蜜橘的危害程度和天敌的控虫能力。主要分析挂果前不同龄期南丰橘树橘叶-柑橘木虱-草间钻头蛛食物链各环节标记物动态变化、相关性和能流。[结果]一定条件下,改良的铕荧光示踪法是一种简便灵敏的橘园生态能流定量测试法,它能较准确地测定南丰蜜橘园食物链中各能流关系。[结论]将改良的铕荧光示踪法用于南丰蜜橘园食物链营养关系的定量分析是可行的。[Objective] The purpose was to deeply understand the trophic relationship among Nanfeng orange,pests and natural enemies and quantitatively describe the population dynamics of pests and natural enemies,and to effectively promote the development of the ecological control technology of insects in Nanfeng Orange Park.[Method] The energy flow in the food chain of orange leaves-Pest-natural enemies in Nanfeng Orange Park were studied by modified fluorescence labeling method using rare earth elements europium as a fluorescent tracer and quantitative analysis of the food chain marks,and the europium content of all trophic levels were determined by biochemical methods such as ashing,coloration,UV absorbance measurement and conversion etc.;The energy flow relationship among all trophic levels was quantized to confirm the harmful degree of pests to Nanfeng orange and the control ability of natural enemies to pests.The dynamic markers,relevance and energy flow of every markers of all links in the food chain of Nanfung orange leaves-Citrus psyllid-Hylyphantes graminicola at different ages before fruit bearing time in Nanfeng Orange were mainly analyzed.[Result] The results showed that under certain conditions,modified fluorescence labeling method using rare earth elements europium as a fluorescent tracer is a simple and sensitive quantitative method of ecological energy flow of Orange Park,and can more accurately determine the relationship of the energy flow of food chain in Nanfeng Orange Park.[Conclusion] It is feasible to using the modified fluorescence labeling method using rare earth elements europium as a fluorescent tracer to quantitatively analyze the trophic relationship of food chain in Nanfeng Orange Park.

关 键 词：稀土元素铕 南丰蜜橘园 生态系统 能流 定量研究 

分 类 号：S666.2[农业科学—果树学]