转甜菜碱醛脱氢酶基因宁夏粳稻幼苗生理研究  

Study on the Physiology of Transgenic Japonica Rice in Ningxia Seedlings with Gene of Betaine Aldehyde Dehydrogenase

作　　者：周晓燕[1,2] 甘晓燕[1] 石磊[1] 宋玉霞[1] 

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]宁夏大学生命科学学院,银川750021

出　　处：《中国农学通报》2012年第6期35-38,共4页Chinese Agricultural Science Bulletin

基　　金：宁夏回族自治区科技攻关宁陕合作项目"抗旱耐盐碱基因克隆;功能分析及应用研究"

摘　　要：为了提高植物耐盐性,充分利用盐碱地,通过采用农杆菌介导法将超旱生耐盐植物梭梭(Haloxylon ammodendron)的甜菜碱醛脱氢酶基因(HaBADH)转入宁夏粳稻,对其阳性植株幼苗进行耐盐性方面的相关生理研究。结果表明,在高浓度NaCl(5.0、7.0g/L)胁迫下,与对照相比,转HaBADH基因粳稻幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性显著提高;丙二醛(MDA)的含量显著降低。说明转入HaBADH基因提高了粳稻对盐胁迫的耐受性。In order to improve plant salt tolerance,full use of saline-alkali soil,through the use of Agrobacterium tumefaciens ultra xeric salt-tolerant plants Haloxylon（Haloxylon ammodendron） of betaine aldehyde dehydrogenase gene（HaBADH） into Japonica rice of Ningxia,its positive seedings to salt tolerance in plants related to physiological research.The results showed that high concentrations of NaCl（5.0,7.0 g/L） stress,compared with the control,transfer HaBADH gene rice seedings superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT） activity was significantly increased;malondialdehyde（MDA） levels were significantly reduced.The results showed that,transfer HaBADH gene could increase Japonica rice of Ningxia tolerance to salt stress.

关 键 词：甜菜碱醛脱氢酶 水稻 转化 耐盐性 

分 类 号：S1[农业科学—农业基础科学]