木薯种质资源遗传多态性SSR分子标记的研究  

Genetic Polymorphism of Cassava Germplasm SSR Molecular Markers

作　　者：彭靖茹[1,2] 马增风[3] 黎萍[1,2] 甘志勇[1] 苏文潘[1,2] 黄惠芳[1,2] 

机构地区：[1]广西壮族自治区亚热带作物研究所,南宁530001 [2]广西壮族自治区木薯研究所,南宁530001 [3]广西农业科学院水稻研究所,南宁530003

出　　处：《中国农学通报》2012年第6期58-62,共5页Chinese Agricultural Science Bulletin

基　　金：广西壮族自治区直属公益性科研院所基本科研业务费专项基金项目"广西木薯种质遗传多态性的研究"(桂热研200803);广西科学研究与技术开发计划项目"高淀粉产量木薯分子育种技术的研究"(主席基金09203-03)

摘　　要：为了研究广西壮族自治区亚热带作物研究所从泰国、菲律宾、缅甸、印度尼西亚等国引进的25份木薯种质资源及海南省和广西壮族自治区亚热带作物研究所等选育的65份木薯品种(系)的遗传多样性,应用12对SSR引物对80个木薯种质进行PCR扩增,获得119个扩增带形,扩增产物的片段大小范围在100～300bp之间,每对引物检测等位基因4～16个,平均为10个。根据品系间的遗传相似系数,利用UPGMA法进行聚类分析,以遗传相似系数0.46为阈值,将80个木薯品系分为2类。聚类分析结果表明,引进的国外资源和所选育的种质资源丰富了中国木薯种质库,拓宽了中国木薯遗传育种的物质基础,为进一步进行木薯新品种的选育和种质资源的收集及管理提供了遗传依据。In order to analyze the genetic diversity in 80 cassava accessions,among which 25 were Guangxi Subtropical Crops Research Institute introduced from Thailand,Philippine,Burma and Indonesian,and 65 varieties belong to Hainan and this unit etc,by using 12 pairs of SSR markers,119 loci were obtained totally,the fragment size of PCR products ranging from 100 bp to 300 bp,4 to 16 alleles were detected by each pair of primer,with an average of 10.According to the genetic similarity coefficient among different strains and the UPGMA method,with the similarity coefficient criteria of 0.46,these 80 accessions were clustered into 2 groups.These results indicated that the introduction of foreign resources and the breeding varieties of Guangxi Subtropical Crops Research Institute enriched cassava germplasm bank in China,and expanded our country＇s cassava breeding basis.It also provided the genetic basis for the breeding of new varieties of cassava or the collection and management of germplasm resources.

关 键 词：木薯 SSR 遗传多样性 聚类分析 

分 类 号：S533[农业科学—作物学]