小麦穗发芽抗性的4个分子标记有效性检验  

Evaluation of Four Molecular Markers Associated with Pre-harvest Sprouting Tolerance in Chinese Wheat

作　　者：赵斌[1] 陈晓东[1] 万映秀[1] 王瑞[1] 张平治[1] 

机构地区：[1]安徽省农科院作物所/安徽省农作物品质改良重点实验室,合肥230031

出　　处：《中国农学通报》2012年第6期115-120,共6页Chinese Agricultural Science Bulletin

基　　金：中央财政拨款项目"现代农业产业技术体系建设专项资金"(CARS-3-2);安徽省科技厅科技平台建设项目"白粒抗穗发芽小麦种质筛选及利用蛋白质电泳鉴定大豆品种研究"(6094035.0);国家转基因生物新品种培育重大专项"优质高低分子量麦谷蛋白亚基转基因小麦新品种选育"(2009ZX08002-014B)

摘　　要：为筛选出适宜黄淮麦区和长江中下游麦区种植的抗穗发芽白粒小麦品种或种质资源,以36份黄淮麦区和长江中下游麦区的主要品种(系)及地方品种为研究对象,对已报道的4个与穗发芽抗性相关的分子标记:Vp1B3、Xgwm155、Xgwm269和Xbarc170进行有效性验证。测定参试材料种子萌发指数(GI),并用上述4种标记进行PCR扩增,对扩增条带进行统计分析。结果表明,GI值显示,红粒品种(GI均值为5.1%)明显较白粒品种(GI均值为28.0%)低;4种标记扩增出的带型中仅Vp1B3的845bp片段能有效地区分36份小麦品种(系);GI值筛选出6份抗穗发芽品种(系)中,其中3份为Vp1B3标记鉴定,可作为黄淮麦区和长江中下游麦区小麦穗发芽抗性育种中首选基因资源。The aim of this study was to assess the efficiency of four markers（Vp1B3,Xgwm155,Xgwm269 and Xbarc170） in identifying wheat genotypes with higher PHS tolerance among 36 Chinese wheat landraces and main varieties（or lines） from Yellow-Huai Facultative Winter Wheat Region and Middle-Lower Yangtze Valley Winter Wheat Region.The germination indexes（GI） value of red wheat（5.1%） was significantly lower than those of white wheat（28.0%）.Among products by the four markers,only 845 bp of Vp1B3 could effectively distinguish 36 wheat varieties（lines）.Three of screened six white wheat cultivars with GI 10%,could be further identified by the marker Vp1B3.They can be used in wheat Pre-harvest sprouting resistance breeding in Yellow-Huai Facultative Winter Wheat Region and Middle-Lower Yangtze Valley Winter Wheat Region.

关 键 词：小麦 穗发芽 分子标记 

分 类 号：S512.1[农业科学—作物学]