半夏与其伪品掌叶半夏的RAPD鉴别  

Identification Pinellia ternata and Pinellia pedatisecta by RAPD

作　　者：杨俊宝[1] 彭正松[2] 

机构地区：[1]川北医学院生物学教研室,四川南充637007 [2]西华师范大学生命科学学院,四川南充637002

出　　处：《现代中药研究与实践》2012年第1期18-20,共3页Research and Practice on Chinese Medicines

基　　金：四川省杰出青年基金后续项目(编号:05ZQ026-041)

摘　　要：目的建立半夏及其伪品掌叶半夏的快速简单的分子鉴定方法。方法 SDS法提取半夏和掌叶半夏的基因组DNA,20个10碱基随机引物(S1-S20,上海生工)用于RAPD-PCR分析。结果 20个引物中有7个有效稳定的引物在半夏和掌叶半夏之间能显示出多态性指纹图谱,其中S10和S17在半夏和掌叶半夏之间显示较大差异。结论利用引物S10和S17进行RAPD-PCR能有效鉴别半夏和掌叶半夏。Obiecfive To establish a rapid and simple molecular identification method for Pinellia ternata and Pinellia pedatisecta. Methods Genomic DNA was isolated from the dried tubers of P ternata and P pedatisecta by modified SDS method. Twenty 10-mer random primers （S1-S20） were used for RAPD analysis. Results Seven efficient and stable primers, showing distinct and polymorphic fingerprints, were selected from the 20 primers. The primers S 10 and S 17 were selected to differentiate Pinellia ternata from Pinellia pedatisecta. Conclusion RAPD analysis using the primer S10 and S17 would be accurate and reliable to identify P. ternata from P pedatisecta.

关 键 词：半夏 掌叶半夏 指纹分析 RAPD 

分 类 号：R931.5[医药卫生—生药学]