苯酚-硫酸法测定苦豆子多糖含量  

Determination of Polysaccharides in Sophora alopecuroides L. by the Method of Phenol-Vitriol

作　　者：邸明磊[1] 单晓菊[1] 马丽娜[1] 张岩[1] 王鹏[2] 陶遵威[3] 

机构地区：[1]天津中医药大学,天津300073 [2]天津医科大学,天津300070 [3]天津市医药科学研究所,天津300020

出　　处：《现代中药研究与实践》2012年第1期59-61,共3页Research and Practice on Chinese Medicines

基　　金：天津市应用基础与前沿技术研究计划重点项目(项目编号:K10JCZDJC21000)

摘　　要：目的建立了苦豆子多糖含量的测定方法，确定了苦豆子多糖的含量。方法用苯酚-硫酸法，在波长490nm处测定吸光度，苦豆子多糖在20-100rμg／mL范围内具有良好的线性关系，相关系数为0．9995。平均回收率为101．4％，RSD=1．98％。结果苦豆子多糖的最佳提取条件为料液比1：10，提取2次，提取时间3h，苦豆子多糖换算因子为1．22，苦豆子中多糖的含量为90．90g／kg。结论该方法简便、准确率高、重现性好，可用于苦豆子中多糖含量的测定。Objective To establish an analysis method for the determination of polysaccharides from Sophora alopecuroides L.Methods The method of phenol-vitriol was used to determine the content of polysaccharide. The wavelength was set at 490 nm.The calibration curve of polysaccharide showed a good linearity within the range of 20-100 μg/mL. The average recovery was 101.4% with RSD=l.98%. Results The optimum extraction condition of polysaccharides was as follows： solid-liquid ratio was 1 ： 10, extracted 2 times and each for 3 h. The converting factor was 1.22 and the content was 90.90g/kg.Conclusiou This method was simple, accurate and repeatable,which could be applied to determine the polysaccharides in Sophora alopecuroides L.

关 键 词：苦豆子 多糖 苯酚-硫酸法 含量测定 

分 类 号：R927.2[医药卫生—药学]